白血病多发家系的遗传因素分析  

Analysis of the hereditary causes in familiar leukemia

作　　者：郝麟[1] 朱平[1] 胡兰[2] 张新[1] 王奕嘉[1] 王逸群[1] 刘英[1] 

机构地区：[1]北京大学第一医院血液学研究室,100034 [2]公安部物证鉴定中心DNA处,100038

出　　处：《中国优生与遗传杂志》2003年第5期3-7,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的　通过一家族多发性白血病家系的基因连锁分析结合国内外文献探讨白血病发病的遗传因素。方法　收集 4代 2 0人中有 3人患有急性粒细胞白血病M1亚型的家系。应用DNAIQ试剂盒从骨髓涂片上提取DNA ,酚氯仿抽提法从外周血提取DNA。逆转录 -多重巢式PCR方法筛查白血病常见融合基因。利用Powerplex试剂盒进行微卫星多重荧光PCR扩增。结果　筛查家系中 5位成员未发现融合基因。确认 3例白血病患者代表位于 11条不同染色体上 11个基因的微卫星没有共同连锁关系。其他 2个微卫星由于在这个家族中的多态性不高 ,无法确认或者排除基因的连锁关系。结论　仅仅根据不充分的家系特征和某些细胞遗传学异常就认定白血病多发家系是遗传性白血病显然还缺乏证据。Objective: Analysis the hereditary causes in familiar leukemia and review the related articles. Methods: Use the DNA IQ System to extract DNA from the marrow smear. Other DNA are prepared from the venous blood by the hydroxybenzene-chloroform extraction method. To apply the reverse transcription multiplex nested polymerase chain reaction to analyze the fusion genes. Microsatellites were amplified by three-color fluorescence PCR with Powerplex system. Results: No fusion gene were detected in five persons in the family. The linkages of 11 locus on 11different chromosomes of the 3 patients were excluded .We can't confirm or exclude the linkages of the other two microstallites. Conclusions: We cannot confirm the familiar leukemia as hereditary leukemia by inconvincible familiar characters and some cytogenetic abnormalities.

关 键 词：家族多发性白血病 遗传因素 微卫星 

分 类 号：R733.7[医药卫生—肿瘤]