消化道恶性肿瘤组织中P糖蛋白、DNA拓扑异构酶Ⅱ及谷胱甘肽转移酶π亚型的表达  

Expression of P-glycoprotein, DNA topoisomerase Ⅱ, glutathione S-transferase π in malignant digestive canal carcinomas

作　　者：张军初[1] 江道振[1] 徐昕昀[1] 何金[2] 刘会敏[2] 

机构地区：[1]第二军医大学长征医院普通外科,上海200003 [2]长征医院病理科

出　　处：《第二军医大学学报》2003年第10期1134-1136,共3页Academic Journal of Second Military Medical University

摘　　要：目的:检测P糖蛋白(P-gp)、DNA拓扑异构酶Ⅱ(TopoⅡ)和谷胱甘肽转移酶π亚型(GsTπ)在消化道恶性肿瘤组织中的表达,并初步探讨其临床意义。方法:用免疫组化法检测P-gp、TopoⅡ和GSTπ在47例消化道恶性肿瘤(食管癌15例,胃癌12例,结直肠癌15例,肝癌4例,胆囊癌1例)组织中的表达,并对其与病理参数的关系进行了研究。结果:(1)在食管癌、胃癌及结直肠癌组织中,P-gp表达阳性率分别为0、16.7％和66.7％;TopoⅡ表达阳性率分别为86.7％、91.7％和100％;GSTⅡ表达阳性率分别为20.0％、25.0％和48.9％。(3)P-gp和GSTπ表达有明显相关性(r=0.979,P<0.05)。(2)P-gp和GSTπ表达的阳性率在胃癌肠型与弥漫型、高和中分化型腺癌与低分化型腺癌之间均无统计学差异(P>0.05);而结直肠癌高分化型腺癌组织中P-gp的表达阳性率显著高于中分化型腺癌(P<0.05)。结论:P-gp、TopoⅡ与GSTπ在食管癌和胃癌的多药耐药表型中的作用有限;结直肠癌的耐药表型与P-gp表达密切相关。Objective: To observe the expression of P-glycoprotein (P-gp), DNA topoisomerase Ⅱ (Topo Ⅱ ) and glu-tathione S-transferase π (GSTπ) in human malignant digestive canal carcinomas. Methods: Expression of P-gp,Topo Ⅱ and GSTπ in tumor tissues of 47 patients with malignant digestive canal carcinomas were determined by immunohistochemical staining. Results: (1) The positive rates of P-gp expression in esophagus,gastric and colorectal carcinomas were 0,16. 7 Mi and 66. 7% respectively,while those of Topo I were 86. 7% ,91. 7% and 100% ,and those of GSTπ were 20. 0% ,25. 0% and 48. 9%. (2)The expression level of P-gp showed a significant relation with that of GSTπ (r=0. 979,P<0. 05). (3) The positive rate of P-gp and GSTπ expression in moderately-differentiated gastric carcinomas showed no statistical difference with that in poorly-differentiated gastric carcinomas .while the positive rate of P-gp expression was significantly higher in well-differentiated colorectal carcinomas than that in moderately-differentiated ones(P<0. 05). Conclusion: It suggests that P-gp, Topo Ⅱ and GSTπ play limited roles in multidrug resistance(MDR) phenotype of esophagus and gastric carcinomas, but P-gp is related to the MDR phenotype of colorectal carcinomas.

关 键 词：消化系统肿瘤 P糖蛋白 DNA拓扑异构酶Ⅱ 谷胱甘肽转移酶π亚型 

分 类 号：R735[医药卫生—肿瘤]