建立人脑胶质瘤恶性进展相关基因表达谱  

Establishment of malignant progression associated gene expression profiles in human brain glioma

作　　者：黄强[1] 董军[1] 王爱东[1] 邵耐远[1] 孙继勇[1] 李晓楠[2] 兰青[1] 胡赓熙[3] 

机构地区：[1]苏州大学附属第二医院神经外科暨脑肿瘤研究室,215004 [2]美国贝勒医学院儿童肿瘤中心 [3]中国科学院上海细胞生物化学研究所

出　　处：《中华肿瘤杂志》2003年第5期437-440,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目 (3 0 0 70 772 )

摘　　要：目的　建立人脑胶质瘤恶性进展相关基因表达谱。方法　用微阵列技术对同一胶质瘤患者初发 (WHOⅡ级 )、复发 (WHOⅢ级 )和再发 (WHOⅣ级 )的 3次手术标本中基因差异表达情况进行检测 ,并以第 3次手术时切取的正常脑组织作为对照。结果　全组共获取 16 36 3个数据。 3份肿瘤标本中 ,表达差异≥ 3倍的基因共 197条。将含正常脑组织在内的 4份标本进行两两比较 ,表达差异≥ 3倍的基因共 4 89条 (上调 193,下调 2 96 )。再将已知功能的 10 9条基因按出现频率排列 ,由高到低依次为发育、代谢、分化、信号传导、DNA结合转录、细胞受体、免疫、DNA合成修复重组、离子通道运输、蛋白翻译合成、细胞骨架运动、应激、原癌和抑癌、细胞凋亡、细胞周期相关基因。结论　从处于恶性进展不同阶段的人脑胶质瘤手术标本中发现了 197条表达差异≥ 3倍的基因 ,同时经生物信息学分析 ,发现 17条候选新基因 ,它们在胶质瘤发生与发展的分子机制中起重要作用。Objective To establish malignant progression associated gene expression profiles in human brain glioma. Methods The primary (WHO grade Ⅱ), recurrent (WHO grade Ⅲ) and re-recurrent (WHO grade Ⅳ) glioma specimens were sequentially collected from one single patient. Gene expression of different tumor specimens and normal brain tissue of the same patient was compared by microarrary techniques. Results 197 differentially expressed genes with differential ratio ≥3 were observed when compared with normal brain tissue. When the specimens (3 tumor, 1 normal brain) were paired with each other, 7 groups containing 489 genes (upregulated 193, downregulated 296) were observed. According to the descending frequency of the 109 genes with known function, they were the genes associated with development, metabolism, differentiation, signal transduction, DNA binding transcription, cellular receptor, immunity, ion-channel transportation, protein translation, cell backbone motion, stress, protooncogene and anti-oncogene and cell apoptosis, respectively. Conclusion From the 197 differentially expressed genes found in one glioma patient experiencing tumor malignant progression, 17 genes screened out by bioinformatics assay, may offer valuable information on molecular mechanisms on genesis and malignant progression of glioma.

关 键 词：脑胶质瘤 基因表达谱 微阵列 脑组织 肿瘤标本 

分 类 号：R739.4[医药卫生—肿瘤]