黄孢原毛皮革菌降解苯胺的工艺研究  

Studies on the Degradation Conditions of Aniline by Phanerochaete chrysosporium

作　　者：刘凌[1] 江振然[1] 胡道伟[1] 常天俊[1] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉湖北430074

出　　处：《生物技术》2003年第5期34-35,共2页Biotechnology

摘　　要：通过正交试验优化筛选了适合黄孢原毛皮革菌降解苯胺的适宜培养基和摇瓶培养降解条件。结果表明 :其适宜降解的液体培养基组成为 :蔗糖 2 0g L ,可溶性淀粉 2 0g L ,(NH4 ) 2 SO4 10g L ,Mn2 + 1μmol L ,Tween- 80 0 .3% ,蛋白胨 30g L。适宜降解的摇瓶培养条件为 :接种量为 2 0 %、pH为 7.0、温度为 30℃、培养时间为 12d。此条件下的苯胺最高降解率可达 95 .5 %。Through orthogonal experiment,the optimal condition was given forthe degradation of aniline by Phanerochaete chrysosporium.The optimum culture conditions of the bacteria were studied,the optimal medium was as follows(g/L):sucrose 20g,amylum 20g,3% Tween-80,(NH_4)_2SO_4 10g,peptone 30g,The optimum growth pH and temperature of the bacteria were 7.0 and 30℃,The highest removal rates of aniline were 95.5%,at 37℃ for 12 days by this condition.

关 键 词：黄孢原毛皮革菌 苯胺 生物降解 环境污染 

分 类 号：X172[环境科学与工程—环境科学] Q939.9[生物学—微生物学]