地塞米松诱发小鼠腭裂时AnnexinⅠ、cPLA_2和PCNA表达的研究  

Study on the expression level of AnnexinⅠ、cPLA_2 and PCNA in cleft-palate mice induced by dexamethasone

作　　者：石冰[1] 左晖[2] 毛祖彝[1] 

机构地区：[1]四川大学华西口腔医学院口腔颌面外科,成都610041 [2]四川省第二人民医院口腔颌面外科

出　　处：《中华口腔医学杂志》2003年第3期188-191,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金(3 9770 80 1);国家教委高等学校优秀教学和科研奖励基金(1999)资助项目

摘　　要：目的　检测膜联蛋白 (AnnexinⅠ )、胞质磷脂酶A2 (cytosolicphospholipaseA2 ,cPLA2 )、增殖细胞核抗原 (proliferatingcellmuclearantigen ,PCNA)在地塞米松诱导小鼠腭裂腭胚突中的表达变化情况。探讨腭裂的发生机制和预防方法。方法　A/J和C5 7BL/ 6 j受孕小鼠随机分为正常对照组、地塞米松致畸组 (DEX组 )、VitB12 致畸拮抗组 (DE +Vit组 )和VitB12 组。应用蛋白质免疫印迹的方法检测AnnexinⅠ、cPLA2 和PCNA的相对含量。结果　A/J小鼠中 ,在正常对照组中 ,随着腭胚突的发育 ,AnnexinⅠ表达逐渐升高 ,其余各实验组也呈相似变化。在DEX组和DEX +VitB12 组中 ,AnnexinⅠ的表达明显较对照组和VitB12 组高 ,对照组与VitB12 组间差异无显著性。而cPLA2 则和PCNA呈相反表达趋势 ,在正常小鼠腭胚突中表达随小鼠发育逐渐下降。在C5 7BL/ 6 j小鼠中 ,正常对照组与DEX组间在AnnexinⅠ和cPLA2 测量差异无显著性。结论　AnnexinⅠ和cPLA2 介导了糖皮质激素诱发腭裂的过程。VitB12 未能阻遇DEX上调AnnexinⅠ的表达 ,但可以对抗DEX抑制cPLA2 的作用 ,这可能是VitB12Objective To test the changing expression level of AnnexinⅠ, cPLA 2 and PCNA in the palatine process of cleft palate mice A/J and C57B/6j induced by dexamethasone To discuss the developing mechanism of cleft palate and the corresponding preventive methods Methods Pregnant mice A/J and C57BL/6j were randomly divided into normal group as blank control,group with deformity induced by dexamethasone,groupgiven VitB 12 as antagonist to deforming factor,group given only VitB 12 The relative quantity of AnnexinⅠ,Cpla2 and PCNA were detected by immunoblotting Results Among mice A/J, with the development of palatine process, AnnexinⅠ′s expression level was increased in the normal group, and other groups showed the similar changes AnnexinⅠ′s expression level was significantly higher in group DEX and group DEX+VitB 12 than in normal group and VitB 12 group of, while there was no significant difference between normal group and VitB 12 group But the changes of cPLA 2 and PCNA expression level was in an opposite direction, with development it decreased in the normal mice′s palatine process In mice C57B/6j there was no significant difference between normal group and group DEX on the measured quantity of AnnexinⅠ, cPLA 2 and Conclusions AnnexinⅠ and cPLA 2 introduce glucocorticoid to induce cleft palate VitB 12 can not inhibit DEX′s enhancing effect on the expression level of AnnexinⅠ, but it can antagonize DEX′s inhibiting effect on expression level of cPLA 2, which is probably one of the mechanisms why VitB 12 antagonize glucocorticoid′s deforming effect

关 键 词：地塞米松 小鼠 腭裂 AnnexinⅠ CPLA2 PCNA 膜联蛋白 胞质磷腊酶A2 增殖细胞核抗原 

分 类 号：R782[医药卫生—口腔医学]