人doc-1蛋白抗原肽的设计  

Design of the antigenic peptide of human doc-1 protein

作　　者：孙文斌[1] 孙沫逸[1] 雷德林[1] 程晓兵[1] 洪咏龙[1] 

机构地区：[1]第四军医大学口腔医学院口腔颌面外科,西安710032

出　　处：《山西医科大学学报》2003年第5期385-386,共2页Journal of Shanxi Medical University

基　　金：国家教育部留学归国人员科研启动基金 (2 0 0 2HG0 0 1)

摘　　要：目的　设计人doc 1蛋白的B细胞连续性抗原表位。方法　根据氨基酸序列利用生物信息学和互联网上蛋白质序列分析软件分析抗原表位 ,包括EMBOSS、COILS 2 .1方案以及亲水性分析、二级结构转角、电荷数、屈曲性分析等 ,确定B细胞连续性抗原表位。结果　doc 1蛋白连续性抗原表位可能位于第 75～ 87、10 8～ 115位氨基酸残基附近。结合其功能特点等原则 ,第 10 8～ 115被确定为合成肽序列。结论　doc 1蛋白B细胞连续性抗原表位的设计 ,为利用合成肽制备抗人doc 1蛋白抗体提供了理论依据 ,也为进一步研究doc 1基因及其蛋白功能奠定了基础。Objective To design the B-cell continuous antigenic peptide of doc-1 protein.Methods By bioinformatics technology and protein sequence analysis software in internet,including EMBOSS, COILS 2.1, hydrophilicity, β-turns, charge prediction and flexbility prediction, the appropriate antigenic peptide sequence was designed according to amino acide sequence (aa No. BAA22937) of human doc-1 protein. Results It showed that the appropriate peptide sequence was probably at or adjacent to 75～87 and 108～115.With its main function,the 108～115 was choosed and synthesized. Conclusion Predictably, the antigenic peptide is helpful to generation of polyAb to human doc-1 protein using the synthetic peptide approach, and it is also a basis to study the doc-1.

关 键 词：人doc-1蛋白抗原肽 设计 氨基酸 生物信息学 蛋白质序列分析软件 抗原表位 口腔肿瘤 

分 类 号：R392-33[医药卫生—免疫学] R739.8[医药卫生—基础医学]