脑胶质细胞瘤VEGF、COX-2基因蛋白表达及临床意义  

Expressions of VEGF, COX-2 Gene Protein in the Gliomas and their Clinical Significance

作　　者：杜继臣[1] 吴宇明[2] 齐风英[3] 左连富[4] 

机构地区：[1]航天中心医院神经内科,北京100039 [2]航天中心医院病理科 [3]河北医科大学病理学与病理生理学教研室 [4]河北省肿瘤研究所

出　　处：《中国误诊学杂志》2003年第10期1460-1463,共4页Chinese Journal of Misdiagnostics

摘　　要：目的:探讨血管内皮生长因子(VEGF)、环氧化酶-2(COX-2)基因蛋白表达在脑胶质瘤发生、生长和转移过程中,以及由低恶性度肿瘤向高恶性度肿瘤的转化中的作用。方法:选用54例Ⅰ～Ⅳ级星形细胞瘤手术后存档蜡块标本为实验标本,选用6例正常脑组织为对照。应用流式细胞术(FCM)分别定量检测Ⅰ～Ⅳ级星形细胞瘤组织中VEGF、COX-2基因蛋白表达量。结果:Ⅰ～Ⅳ级星形细胞瘤VEGF蛋白表达阳性率分别为23.1％、46.2％、100％和100％。COX-2蛋白在星形细胞瘤中的表达量(FI值)增加,并随肿瘤恶性度的升高而增加。COX-2蛋白表达量FI值与病理组织学分级有明显相关性(P<0.01)。COX-2蛋白表达阳性率分别为78.6％、85.7％、100％和100％。COX-2蛋白表达量(FI值)与VEGF表达量(FI值)相关分析显示二者呈显著正相关(r=0.54178,P<0.001)。结论:在星形细胞瘤形成以及肿瘤由低恶性度向高恶性度演化过程中,VEGF的过度表达具有重要作用;VEGF表达的水平是预测临床预后的重要标志。Objective:To investigate the expressions of vascular endothelial growth factor (VEGF), COX-2 gene protein in gliomas, and to evaluate their roles in tumorigenesis, growth, invasion and metastasis. Methods;The experimental group contained 54 paraffin-embeded samples from patients with astrocytoma brain tumors, including 13 cases of grade Ⅰ , 13 grade Ⅱ, 14 grade Ⅲ and 14 grade IV. 6 cases of normal brain tissues were taken as control group. The expressions of VEGF, COX-2 gene protein in astrocytoma were detected quantitatively by Flow Cytometry(FCM). Results ;The positive expression rates in astrocytoma of grade Ⅰ,Ⅱ,Ⅲ and IV were 23.1%, 46. 2% , 100% and 100% respectively. The FI of COX-2 protein in astrocytoma was higher and correlated with increasing histological grade (P< 0. 01). The positive rates expression in astrocytoma of grade I,II,III and IV were 78. 6% , 85. 7% , 100% and 100% respectively. The FI of COX-2 protein was correlated significantly with the FI of VEGF protein (r = 0. 54178,P< 0. 001). Conclusion:These findings indicate that overexpression of VEGF protein in tumor cell should play an important role in the tumorigenesis and malignant transformation. The high level of VEGF might be a strong predictor of poor survival.

关 键 词：神经胶质瘤/病理学 淋巴因子/遗传学 内皮生长因子/遗传学 环加氧酶抑制药/遗传学 基因表达 漉式细胞术 

分 类 号：R739.264[医药卫生—肿瘤]