Degradation of pyrene and characterization of Saccharothrix sp.PYX-6 fromthe oligotrophic Tianchi Lake in Xinjiang Uygur Autonomous Region,China  

作　　者：HUYuting RENFenghua ZHOUPeijin XIAMin LIUShuangjiang 

机构地区：[1]InstituteofMicrobiology,ChineseAcademyofSciences,Beijing100080,China [2]BeijingCenterforPhysicalandChemicalAnalysis,Beijing100089,China

出　　处：《Chinese Science Bulletin》2003年第20期2210-2215,共6页

基　　金：supported by the National Natural Science Foundation of China(Grant No.2017734);the Knowledge Innovation Program(Grant No.KSCX2-SW-113,KZCX1-SW-12-Ⅱ-02)of CAS

摘　　要：Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsmorphology, physiological and biochemical properties, cellwall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 wassequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was namedSaccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilatedpyrene for growth and 0.005% of yeast extract stimulatedpyrene degradation and assimilation. The optimal pH ofcultural medium and the optimal shaking frequency during cultivation were 6—8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacteriumsp. PYR-1.Bacterial strain PYX-6 that utilizes anthracene, phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomons Region of China. Itsmorphology, physiological and biochemical properties, cellwall pattern and G+C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 wassequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was namedSaccharothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilatedpyrene for growth and 0.005% of yeast extract stimulatedpyrene degradation and assimilation. The optimal pH ofcultural medium and the optimal shaking frequency during cultivation were 6—8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp. PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacteriumsp. PYR-1.

关 键 词：中国 新疆 天池 湖泊污染 PYX-6菌株 核退化 形态学 生理学 生物化学性质 

分 类 号：X172[环境科学与工程—环境科学]