行为训练与小鼠海马突触蛋白磷酸化水平的相关性  

CORRELATION BETWEEN SYNAPTIC PROTEIN PHOSPHORYLATION IN HIPPOCAMPI AND SHUTILE BOX AVOIDANCE BEHAVIOR

作　　者：高静[1] 吴馥梅[1] 萧信生[1] 王新光 

机构地区：[1]南京大学生物系生理教研室,南京210008 [2]南京大学现代分析中心

出　　处：《生理学报》1992年第1期86-91,共6页Acta Physiologica Sinica

摘　　要：本文对梭箱回避行为训练是否能改变小鼠海马突触蛋白磷酸化水平进行了检定。在小鼠训练结束的不同时程制备突触体,以γ-^(32)P-ATP作为标记进行离体测定。结果如下:(1)正常小鼠各脑区突触蛋白磷酸化水平无显著差异;(2)学习训练刚结束时,海马突触蛋白磷酸化水平与学习训练成绩的优劣有相关性;(3)学习训练结束后24h,上述相关性更为明显,且成绩良好组右侧海马突触蛋白磷酸化水平显著高于左侧,而成绩低劣组相反。海马突触蛋白磷酸化的这种不对称现象的成因尚不清楚。这些结果表明,海马突触蛋白磷酸化反应可能是习得行为的分子机制。The present investigation was undertaken to detect whether protein phos-phorylation levels of hippocampal synaptosomes in mice trained on shuttle boxavoidance task would show some changes. The synaptosomes were prepared at different times after the conclusion oftraining and labelled with γ-^(32)P-ATP for in vitro determination. The results are asfollow: (1) No significant differences were found in the phosphorylation of syn-aptosomal proteins in different brain areas in normal mice before training. (2)Immediately after the conclusion of shuttle box avoidance task there appears acorrelation between protein phosphorylation levels in hippocampal synaptosomesand the degree of success of training. (3) 24 h after the completion of training theabove relationship is more significant, and protein phosphorylation levels of righthippocampus in Good Score Group is markedly higher than that of left one, butin the Inferior Score Group, the left-right difference is reverse. The formationof such asymmetry in protein phosphorylation in hippocampi is unknown. Thesedata indicate that the synaptic protein phosphorylation in hippocampi might bea molecular mechanism of acquisition.

关 键 词：海马 突触蛋白 行为训练 磷酸化 

分 类 号：Q426[生物学—神经生物学]