分泌抗黄曲霉毒素M1抗体的大鼠-小鼠淋巴细胞杂交瘤细胞株的建立及其特性的研究  

Establishment of Rat-mouse Lymphocyte Hybridoma Clones Secreting Anti-aflatoxin M1 Antibodies and the Properties of the Secreted Antibodies

作　　者：季永镛[1] 林国妹 叶敏[1] 

机构地区：[1]中国科学院上海细胞生物学研究所

出　　处：《生物工程学报》1992年第2期150-156,共7页Chinese Journal of Biotechnology

摘　　要：通过大鼠、小鼠对AFM1-BSA抗体应答的比较,选用应答强的大鼠脾细胞作为亲本细胞,与小鼠骨髓瘤P3X63-Ag8.653系细胞融合制作杂交瘤。经HAT培液选择和RIA的筛选,克隆化,最终获得生长良好、稳定分泌抗AFM1抗体的5株大鼠-小鼠淋巴细胞杂交瘤细胞株。以ELISA,竞争结合的RIA进一步证明获得的5个单抗是针对AFM1,并与其衍生物AFB1有明显的交叉反应。5个单抗亲和力的平衡常数K为10^+—10^(11) l/M,这表明这些单抗具有组建检测AF试剂盒的潜在实用价值。Through the comparison of antibody responses to AFM1-BSA between rats and mice,rat spleen cells were chosen as a partner to fuse with mouse myeloma cell line P3X63-Ag8.653 cells to construct hybridoma. The radioimmunoassay for the detection of specific anti-AFM1 antibodies designed in our lab was used to screen hybridoma cells. Five clones of hybridomas secreting anti-AFM1 antibodies were obtained, named as clone 1C6, 1E6, 2E4, 3E2 and 4E1. Their specificity against AFM1 was further characterized with other antibody assays different from that used in screening. The results showed that in addition to their specificity for AFM1 the five monoclonal antibodies all cross-reacted with AFB1 significantly. The equilibrium constant K of antibody affinity of the five clones was 10~9—10^(11)l/M. It indicated the potentiality of their application to produce aflatoxin immunodetection kit.

关 键 词：单克隆抗体 黄曲霉毒素 

分 类 号：R392.2[医药卫生—免疫学]