丙酰螺旋霉素基因工程菌构建的研究  

Construction of Genetic Engineering Strain Producing Propionylspiramycin

作　　者：李元[1] 刘伯英[1] 李晓平[1] 杨毓芬[1] 王硕昌 

机构地区：[1]中国医学科学院医药生物技术研究所

出　　处：《生物工程学报》1992年第2期128-133,共6页Chinese Journal of Biotechnology

摘　　要：将含有丙酰化酶基因的pIJM9重组质粒转化至螺旋霉素生产菌株S.spiramyceticus获得的转化子,经菌落原位杂交和Southern分子杂交表明,No.61转化子含有pIJM9重组质粒,其发酵产物经薄层层析,生物显迹试验证明,含有与丙酰螺旋霉素标准品Rf值相类似的组分,高压液相色谱分析也证明其产物中有与丙酰螺旋霉素保留时间基本一致的成份,质谱分析结果表明产物中含有与丙酰螺旋霉素Ⅱ相同的组分,这说明No.61克隆菌株是能够直接产生丙酰螺旋霉素的基因工程菌。Our work has demonstrated that the propionylacylase gene cloning and expression of S.mycarofaciens mutant in S.lividans TK54. In this paper, we report the transformation of pIJM9 recombinant plasmid containing propionylacylase gene into spiramycin producer S.spiramyceticus. The results of conoly hybridization and Southern hybridization showed that No.61 transformant habour the pIJM9 recombinant plasmid among the transformants. The TLC and bioautography showed that the Rf value of one component of No.61 transformant fermentation products is similar with propionyl spiramycin. The HPLC retation time of components of No.61 transformant fermentation product and propionylspiramycin are similar too.(FAB)-MS (m/Z943 M+) result showed that there is propionylpiramycinⅡ in the fermentation product of No.61 transformant. According to these results, No.61 transformant is a genetic engineering strain producing propionylspiramycin.

关 键 词：丙酰螺旋霉素 转化 丙酰化酶基因 

分 类 号：R394.8[医药卫生—医学遗传学]