内皮抑制素通过抑制基质金属蛋白酶-2而抑制血管新生(英文)  

Endostatin inhibits angiogenesis by suppressing matrix metalloproteinase-2

作　　者：欣坚[1] 马小超[1] 邓辉[2] 张婷[1] 屠曾宏[1] 

机构地区：[1]中国科学院上海生命科学院 [2]复旦大学附属华山医院皮肤病研究室,上海200040

出　　处：《中国药理学与毒理学杂志》2003年第5期389-394,共6页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的　研究内皮抑制素抑制血管新生作用的机制。方法　用碱性成纤维细胞生长因子 (bFGF)处理体外原代培养人脐静脉内皮细胞 ;用三维胶原模型研究bFGF诱导的血管新生作用 ;用明胶电泳和蛋白印迹法研究分泌于培养基中的基质金属蛋白酶的活性和含量 ;RT PCR法研究基质金属蛋白酶的mRNA水平。结果　内皮抑制素显著抑制bFGF诱导的内皮细胞血管新生能力 ;抑制内皮细胞分泌的基质金属蛋白酶 2的表达及其mRNA的水平。结论　内皮抑制素通过抑制基质金属蛋白酶AIM To study mechanisms involved in antiangiogenic effect o f endostatin. METHODS Human umbilical vein endothelial cells (HUV EC) were isolated and treated with basic fibroblast growth factor (bFGF). 3D-co llagen gel model was used to determine the angiogenesis of HUVEC. Activity of ma trix metalloproteinase (MMP) in culture medium was analyzed by gelatin zymograph y and Western blot. RT-PCR was used to determine mRNA levels of MMP. RESU LTS Endostatin inhibited the angiogenesis of HUVEC induced by bFGF. Endos tatin inhibited the expression and the mRNA level of MMP-2 in a concentration- dependent manner. CONCLUSION MMP-2 inhibition plays an importa nt role in antiangiogenic effect of endostatin.

关 键 词：内皮抑制素 成纤维细胞生长因子 碱性 脐静脉 基质金属蛋白酶 抗血管新生 

分 类 号：R96[医药卫生—药理学]