Expression of Hantaan virus 26 kD fragment of nucleocapsid protein in insect cells and prelimimary study on its immunogenicity  

作　　者：罗雯 张芳琳 阎岩 吴兴安 刘勇 白文涛 王海涛 徐志凯 

机构地区：[1]Department of Microbiology,Fourth Military Medical University,Xi'an 710033,China [2]Department of Microbiology,Fourth Military Medical University,Xi'an 710033,Chinabjective: To express the 26 kD fragment of Hantaan virus nucleocapsid protein that contains the major an-tigenic epitopes in insect cells, and make a preliminary analysis of its immunological characteristics. Methods: The re-combinant baculovirus bac-S0. 7 with the 700 bp fragment of S gene 5'terminal of Hantaan virus was constructed, and the antigenicity of the expression product was tested. Mice were injected with Sf9 cells infected by the recombinant baculovirus. The humoral and cellular immunological effects were identified by indirect immunofluorescence assay, micro-cell culture neutralization test and T lymphocytes stimulation test. Results: Immunized by bac-S0. 7 infecting insect cells, specific antibody with the highest titer of 1:1 600 was observed. The stimulation indexes of splenocytes of immunized mice to nucleocapsid protein of Hantaan virus was higher than the negative control. Conclusion: The expression product of S0. 7 gene fragment in insect cells is immunogenic.

出　　处：《Journal of Medical Colleges of PLA(China)》2003年第5期267-272,共6页中国人民解放军军医大学学报（英文版）

基　　金：National Natural Science Foundation of China (No.30070686);Chinese Educational Deputy Fund for skeleton teachers

摘　　要：Objective: To express the 26 kD fragment of Hantaan virus nucleocapsid protein that contains the major antigenic epitopes in insect cells, and make a preliminary analysis of its immunological characteristics. Methods: The recombinant baculovirus bac-S0.7 with the 700 bp fragment of S gene 5' terminal of Hantaan virus was constructed, and the antigenicity of the expression product was tested. Mice were injected with Sf9 cells infected by the recombinant baculovirus. The humoral and cellular immunological effects were identified by indirect immunofluorescence assay, micro-cell culture neutralization test and T lymphocytes stimulation test. Results: Immunized by bac-S0.7 infecting insect cells, specific antibody with the highest titer of 1∶1 600 was observed. The stimulation indexes of splenocytes of immunized mice to nucleocapsid protein of Hantaan virus was higher than the negative control. Conclusion: The expression product of S0.7 gene fragment in insect cells is immunogenic.Objective: To express the 26 kD fragment of Hantaan virus nucleocapsid protein that contains the major an-tigenic epitopes in insect cells, and make a preliminary analysis of its immunological characteristics. Methods: The re-combinant baculovirus bac-S0. 7 with the 700 bp fragment of S gene 5′terminal of Hantaan virus was constructed, and the antigenicity of the expression product was tested. Mice were injected with Sf9 cells infected by the recombinant baculovirus. The humoral and cellular immunological effects were identified by indirect immunofluorescence assay, micro-cell culture neutralization test and T lymphocytes stimulation test. Results: Immunized by bac-S0. 7 infecting insect cells, specific antibody with the highest titer of 1:1 600 was observed. The stimulation indexes of splenocytes of immunized mice to nucleocapsid protein of Hantaan virus was higher than the negative control. Conclusion: The expression product of S0. 7 gene fragment in insect cells is immunogenic.

关 键 词：Hantaan virus nucleocapsid protein insect cell IMMUNOGENICITY 

分 类 号：R373[医药卫生—病原生物学] R392[医药卫生—基础医学]