低浓度MNNG激活转录因子  

Activation of transcription factors induced by low concentration of N-methyl-N'- nitro- N- nitrosoguanidine

作　　者：王谷亮[1] 王政[1] 杨军[1] 余应年[1] 

机构地区：[1]浙江大学医学院病理生理教研室,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2003年第5期390-392,共3页Journal of Zhejiang University(Medical Sciences)

基　　金：国家重点基础研究发展规划 (973)项目(2 0 0 2 CB5 12 90 1)

摘　　要：目的 :为了进一步研究 N-甲基 - N’-硝基 - N-亚硝基胍 (MNNG)引起的非定标性突变的发生机制 ,观察了低浓度 MNNG对转录因子 NF- κB、CREB、AP- 1和 c- Myc的影响。方法 :采用分泌型碱性磷酸酶报告基因和瞬时转染实验检测转录因子活性。结果 :经 0 .2 μmol/L MNNG处理后 ,转录因子 CREB活性是溶剂对照组的 1.4倍 (P<0 .0 5 ) ,AP- 1和 NF- κB活性均为溶剂对照组的 1.3倍 (P<0 .0 5 ) ;而 c- Myc的活性在对照组和 MNNG处理组都较低。结论 :低浓度 MNNG可以激活转录因子 AP- 1、CREB和 NF- κB,对 c- Myc则无明显激活作用 ,提示一些转录因子的激活可能参与了 MNNG引起的非定标性突变发生过程。Objective: To investigate the effect of MNNG on some of the transcription factors such as NF-κB, CREB, AP-1 and c-Myc. Methods: The activities of these transcription factors were measured by transient transfection assay of SEAP vectors. Results: The expressions of AP-1, CREB and NF-κB driven reporter genes were elevated for about 1.3, 1.4 and 1.3 times in MNNG-treated cells, respectively, as compared to untreated controls. However, the exposure of MNNG had no effect on the activity of c-Myc. Conclusion: The activation of certain transcription factors might be involved in the process of untargeted mutation induced by low concentration of MNNG treatment.

关 键 词：N-甲基-N'-硝基-N-亚硝基胍 转录因子 信号传递 

分 类 号：R363[医药卫生—病理学]