焦炉工尿中1-羟基芘水平与早期遗传学效应的关系  

A study on the relationship between urinary 1-hydroxypyrene level and early genetic effect among coke oven workers

作　　者：冷曙光[1] 郑玉新[1] 戴宇飞[1] 牛勇[1] 张文众[1] 王雅文[1] 李晓华[1] 潘祖飞[2] 肖军[2] 王忠旭[3] 李涛[1] 何凤生[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]辽宁省本溪钢铁集团有限责任公司劳动卫生研究所 [3]辽宁省鞍山钢铁集团公司劳动卫生研究所

出　　处：《中华预防医学杂志》2003年第5期327-330,共4页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助项目 ( 3 9970 63 3 );国家重点基础研究规划资助项目 ( 2 0 0 2CB5 12 90 3 )

摘　　要：目的　研究焦炉工尿中 1 羟基芘水平与外周血淋巴细胞胞质分裂阻滞微核和彗星尾矩间的关系。方法　分别选取 133名焦炉作业工人和 2 8名非职业暴露人员 ,使用碱水解 高效液相色谱法测定尿中 1 羟基芘浓度作为个体多环芳烃暴露内剂量 ,分别应用彗星试验和胞质分裂阻滞微核法评价了研究对象外周血淋巴细胞DNA和染色体损伤 ,使用调查表收集职业暴露史、年龄、性别、吸烟和饮酒状况等信息。结果　调整了年龄、性别、吸烟和饮酒状况后 ,尿中 1 羟基芘浓度与外周血淋巴细胞微核细胞率和彗星尾矩均存在良好的相关关系。进一步按尿中 1 羟基芘水平将研究对象分成0 30～ 2 4 4 (5 4人 )、2 4 5～ 7 0 9(5 3人 )和 7 10～ 33 10 μmol/mol肌酐组 (5 4人 ) 3个内剂量组 ,3组个体尿中 1 羟基芘的几何均值分别为 1 14、4 32和 12 4 9μmol/mol肌酐。使用多元非参数协方差分析校正了不同内剂量组个体的年龄、性别、吸烟和饮酒状况后 ,7 10～ 33 10 μmol/mol肌酐组的彗星尾矩(中位数 3 6 7)显著性高于 2 4 5～ 7 0 9和 0 30～ 2 4 4 μmol/mol肌酐组 (中位数分别为 1 6 8和 1 12 ) ,但后 2组之间差异无显著性 ;2 4 5～ 7 0 9和 7 10～ 33 10 μmol/mol肌酐组微核率的中位数分别为 8 0 0‰和 7 5 0‰ ,2组间?Objective To investigate the relationship between the urinary 1-hydroxypyrene level and cytokinesis-block micronucleus and the olive moment of comet assay in peripheral blood lymphocyte in coke oven workers. Methods One hundred and thirty-three workers from a coke plant and 28 referents without occupational PAH exposure were recruited in this study. Urnary level of 1-hydroxypyrene was measured by alkaline hydrolysis combined with high performance liquid chromatography as an internal exposure dose, and the DNA and chromosomal damage of peripheral blood lymphocyte were evaluated with comet assay and cytokinesis-block micronucleus method. Personal information including occupational history, age, sex, smoking and alcohol drinking, was collected by questionnaire. Results There existed a good correlationship between the urinary level of 1-hydroxypyrene and frequency of micronuclei per 1 000 binucleated cells or the olive moment of comet assay in the study subjects, after adjusting for sex, age, smoking and alcohol drinking (r>0.25, P<0.01). One hundred and sixty-one subjects were divided into three groups by their urine 1-hydroxypyrene level (expressed as 0.30—2.44,2.45—7.09 and 7.10 —33.10 μmol/mol Cr), and the geometric means of their urinary levels of 1-hydroxypyrene were 1.14,4.32 and 12.49 μmol/mol Cr, respectively. After adjusting for age, sex, smoking and alcohol drinking by multiple nonparametric analysis of covariance, the median of olive moment of comet assay in the group of 7.10—33.10 μmol/mol Cr was 3.67, significantly higher than that in the groups of 0.30—2.44 and 2.45—7.09; and the micronuclei frequencies in the groups of 2.45—7.09 and 7.10—33.10 μmol/mol Cr were 8.00‰ and 7.50‰, respectively, significantly higher than that in the group of 0.30—2.44μmol/mol Cr (6.00‰). Conclusions The comet assay of peripheral blood lymphocyte was more suitable to detect the PAHs-induced early genotoxicity, than the cytokinesis-block micronucleus.

关 键 词：焦炉工 尿 1-羟基芘 早期 遗传学 职业暴露 多环芳烃 

分 类 号：R131[医药卫生—劳动卫生]