阿尔茨海默病与血管性痴呆基底细胞微管蛋白变化研究  

Tubulin expression of basal cells in patients with Alzheimer's disease and vascular dementia

作　　者：肖计划[1] 夏潮涌[2] 贾艳滨[1] 郑佩娥[3] 

机构地区：[1]暨南大学医学院精神医学教研室,广东广州510632 [2]暨南大学医学院组织胚胎学教研室,广东广州510632 [3]暨南大学医学院病理解剖学教研室,广东广州510632

出　　处：《中国病理生理杂志》2001年第7期632-634,T002,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No 395 70 2 6 7);广东省重点科技攻关项目 (No 99M0 470 3G)

摘　　要：目的 :研究阿尔茨海默病与血管性痴呆皮肤基底细胞内微管蛋白的数量变化特点以及这些特点作为早期诊断Alzheimer病生物学指标的意义。方法 :使用免疫组化LSAB法对Alzheimer病和血管性痴呆患者皮肤基底细胞α -微管蛋白进行检测 ,用图象分析仪进行定性与吸光度的定量分析。结果 :Alzheimer病组的积分吸光度(IA)为 2 6 1 43± 2 5 2 1、平均吸光度 (AA)为 1.89± 0 14,分别非常显著地高于血管性痴呆组的IA(12 0 .5 5± 19 71)和AA(0 .85± 0 14) ;P <0 0 1。结论 :提示基底细胞微管蛋白的定性与定量分析 ,有可能为老年性痴呆的早期诊断提供一种非神经系统的生物学诊断指标。AIM: To investigate quantitatively the tubulin expression of basal cells in patients with Alzheimer's disease(AD) and vascular dementia(VD) and evaluate its significance in diagnosing Alzheimer's disease. METHODS: α-tubulin of basal cells was examined using LSAB immunohistochemical technique, and analyzed qualitatively and quantitatively by image analyzer. RESULTS: The integrated absorbance (I A ,261.43±25.21) and the average absorbance (A A ,1.89±0.14) in the AD group were significantly higher than those (I A ,120.55±19.71 and A A , 0.85±0.14, respectively)in the VD group ( P< 0.01). CONCLUSION: These data suggest that the α-tubulin of basal cells could be served as a extraneuronal biological marker in diagnosing Alzheimer's disease early. [

关 键 词：阿尔茨海默病 血管性痴呆 基底细胞微管蛋白 老年性痴呆 诊断 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R749.13[医药卫生—临床医学]