酒精对鼠胚胎神经胶质细胞c-fos基因表达的影响  

The effect of alcohol on c-fos gene expression in rat embryo neuroglial

作　　者：屈卫东[1] 肖帮良[2] 吴德生[1] 魏大鹏[3] 翁贵武[1] 谢芳莉[1] 

机构地区：[1]华西医科大学公共卫生学院环境卫生学教研室 [2]华西医科大学公共卫生学院卫生毒理学教研室 [3]华西医科大学公共卫生学院免疫学教研室

出　　处：《中华预防医学杂志》2001年第1期55-56,共2页Chinese Journal of Preventive Medicine

摘　　要：目的　探讨酒精致脑发育异常机制。方法　从孕 19d鼠胚胎脑组织分离培养星形、少突胶质细胞体外分别施不同剂量酒精及其代谢产物乙醛 ,应用免疫细胞化学技术研究二者作用不同时间对星形、少突胶质细胞原癌基因c fos表达影响。结果　酒精对星形、少突胶质细胞c fos表达与时间和剂量有关。各剂量酒精、乙醛作用 1h既可影响两种细胞c fos基因表达 ,2h表达至峰值 ,72h恢复正常呈现特异时相性。结论　酒精、乙醛均可致星形、少突胶质细胞c fos表达增强。cObjective This paper is aimed to explore the mechanisms of brain development abnormality induced by alcohol. Methods Astrocytes and oligodendrocytes of 19 day rat embryo were exploited and cultured in vitro,and alcohol and its metabolite product (acetaldehyde) were added to DMEMF 12 medium After different exposure times,c fos expression of astrocytes and oligodendrocytes was measured by the immunocytochemistry technique. Results Changes in c fos gene expression induced by alcohol and acetaldehyde was time and dose dependent After 1 hr exposure,alcohol and acetaldehyde affected c fos gene expression in two kinds of neuralglia C fos positive expression reached peak value after 2 hr,but recovered after 72 hr and showed special time phase expression. Conclusions Alcohol and acetaldehyde cause abnormal increase of c fos gene expression in astrocytes and oligodendrocytes This abnormal expression may play an important role in abnormal brain development induced by alcohol

关 键 词：酒精 鼠 神经胶质细胞 C-FOS 基因表达 少突神经胶质 胚胎发育 孕期 

分 类 号：R363[医药卫生—病理学]