外生型肝癌细胞系(EGHC-9901)的建立  

ESTABLISHMENT OF HUMAN EXTRAHEPATIC GROWING HEPATOCELLULAR CARCINOMA CELL LINE EGHC-9901

作　　者：吴小鹏[1] 王占民[1] 刘博[1] 刘军[1] 高英茂[2] 李兆亭[1] 刘春生[3] 

机构地区：[1]山东医科大学附属医院普外科,山东济南250012 [2]山东医科大学组织胚胎学教研室,山东济南250012 [3]山东医科大学附属医院肿瘤中心,山东济南250012

出　　处：《中国现代普通外科进展》2000年第2期23-25,共3页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 介绍外生型肝癌细胞系的建立及对其生物学特性测定的结果.方法 将手术切除的肿瘤标本进行体外培养,观察肝癌细胞形态学改变,进行核型分析、增殖动力学、流式细胞计数、裸鼠成瘤情况及AFP测定.结果 外生型肝癌细胞系(EGHG-9901)无论形态学观察,还是功能学测定,均符合肝癌细胞的一般特征,并具有体外分泌AFP功能.肝癌细胞之间形成完整毛细胆管,内有明显微绒毛.结论EGHC-9901细胞系符合外生型肝癌细胞系的特征.To establish a new extrahepatic growing hepatocellular carclnoma cell line. Methods: A speciment that was pathologically identified extrahepatic growing hepatocellular carcinoma were cultured in vitro. The morphology,karyotype analysis, DNA analysis, the tumor formation of heterotransplantation were observed. Results: Morphological observation and functional analysis showed that it had the comnnon features of HOC.It was found to be able to secret AFP in vitro. Conclusion: EGHC-9901 has characteristic of the extrahepatic growing hepatocellular carcinoma cell line.

关 键 词：外生型肝癌细胞系 肝细胞 肿瘤细胞 细胞培养 EGHC-9901细胞系 

分 类 号：R73-351[医药卫生—肿瘤] R735.7[医药卫生—临床医学]