McAb共刺激PBLs细胞超微结构的变化和作用肝癌细胞时间的选择  

Change of PBLs ultrastructure Co - stimulated by McAb and Selection of time acting to hepatoma cell.

作　　者：符生苗[1] 明利华[2] 邓立群[1] 黄树林[3] 

机构地区：[1]海南省人民医院医学研究中心,海口570311 [2]中国医学科学院分子肿瘤国家重点实验室分子免疫室,北京100021 [3]广东药学院分子生物学研究室,广州510224

出　　处：《中国热带医学》2001年第2期100-102,共3页China Tropical Medicine

摘　　要：目的 根据ＭｃＡｂ共刺激 ＰＢＬｓ在不同抗体和不同时间里淋巴细胞的形态变化，找出免疫治疗的最佳时间。方法 用ＭｃＡｂ抗ＣＤ３，ＣＤ２８＋ＣＤ８０激活健康人的ＰＢＬｓ，对各组不同时间段的淋巴细胞超微结构进行观察，并将淋巴细胞作用于肝癌细胞后ＢＥＬ－７４０２，结果 ＣＤ３及 ＣＤ２８＋ＣＤ８０除刺激淋巴细胞增殖外，也能使淋巴细胞活化，细胞表现为胞体增大，细胞器增多，具有粗大的绒毛和突出伪足，并可见单核细胞吞噬活跃；ＣＤ２８＋ＣＤ８０ ＭｃＡｂ组ＰＢＬｓ ７２ｈ后作用 ＢＥＬ－７４０２肝癌细胞出现典型的凋亡现象。结论 本研究对肿瘤的Ｔ细胞治疗有重要意义，为肿瘤生物治疗用药时机提供依据。Objective Morphological change of activated lymphocytes in peripheral blood of healthy person (PBLs) stimulated by McAb against CD3 and CD28 + CD8O, selection of the best time for immunotherapy. Method The PBLs were activated by McAb against CD3 and CD28 ± CD80 and the ultrastructure of PBLs in different study groups and different period were observed under electron microscope, and the PBLs were cocultured with hepatoma cells. Results The results showed that CD3 and CD28 ± CD8O could not only stimulate the proliferation of lymphocytes but could also activate lymphocytes. The bulk of lymphocytes became bigger, cell organ manifolded, the thick villi and protruded were seen and the mononuclear leucocyte was active at manifolded, the thick villi and protruded pseudopod were seen and the mononuclear leucocyte was active at macrophaging. In addition, typical apoptosis were observed in BEL -74-2 hepatoma cells cocultured with PBLs 72 hr after stimulated by CD28 + CD80. Conclusion The study is of significance in immunotherapy of hepatoma and it can provide molecular basis for tumor biotherapy and selection of medicinal.

关 键 词：McAb 共刺激 PBLS 细胞超微结构 肝癌 淋巴细胞 免疫治疗 

分 类 号：R735.7[医药卫生—肿瘤]