P糖蛋白表达在儿童急性白血病中的意义  

THE CLINICAL AND BIOLOGICAL SIGNIFICANCE OF P-GLYCOPROTEIN EXPRESSION IN CHILD ACUTE LENKEMIA

作　　者：何海龙[1] 王津媛[1] 紫忆欢[1] 李祯萍[1] 朱玲琍[1] 

机构地区：[1]苏州医学院附属儿童医院,215003

出　　处：《中国小儿血液》2001年第4期145-147,共3页China Child Blood

摘　　要：为探讨儿童急性白血病 (AL)P糖蛋白 (P gp)表达与临床、免疫分型及预后。运用单克隆抗体UIC2 及流式细胞仪 (FCM)间接免疫荧光法对不同时期儿童AL的P gp表达进行检测。结果 :4 5例初治急性淋巴细胞性白血病 (ALL)P gp表达率8 89% ,1 2例复发ALL阳性率为 2 5% ,完全缓解 (CR)组为 0。 32例初治急性非淋巴细胞白血病 (ANLL)P gp表达率为 1 8 75% ,6例复发ANLL 33 3% ,CR组为 0。初治ANLL患儿P gp表达与CD34 、CD7高度相关 ,初治ALL与CD抗原无明显相关性。P gp+ANLL的CR率 2 0 % ,明显低于P gp ANLL( 83 3% ) ,P gp+ALL的CR率 ( 75% )与P gp ALL的CR率 ( 92 86 % )相近 (P >0 0 5) ,但复发率高。结果表明 :P gp高表达是导致儿童AL化疗耐药的重要机制 ,检测P gp是判断预后 ,指导治疗的有用指标。Objective: To evaluate the Clinical and biological significance of P glycopeotein(P gp) expression in Child Acute Leukemia(AL).Methods: P gp was detected by using monoclonal antibody UIC2 and Folw cytometry. Results: P gp was expressioned in 8.89% of 45 previouly untreated All cases, Being less than in 25.00% of 12 relapsed all case. P gp was expressioned in 18.75% of 32 previouly untreated ANLL cases, Being less than in 33.33% of 6 relapsed ANLL cases, P gp expression was not found in patients at remission lincluding ALL and ANLL. Immunologically, P gp expression was significantly associated with CD 34 CD 7 in ANLL, But no association between CD and P gp expression in ALL. 20% of P gp postive expression previouly untreated ANLL (not including M 3) achived completed remiession, this was sigmificantly lower than 72.2% in P gp negative ANLL (P<0.05) the completeted remission rate was same in P gp positive ALL and P gp negutive ALL(P>0.05), but a higer rate of redapse among P gp postive compared withs P gp negative patients with ALL. Conclosion these sugested that high P gp expression was contribute to the clinical drug resistance and P gp is index of poor prognosis in child AL.

关 键 词：P糖蛋白 儿童 急性白血病 免疫分型 预后 

分 类 号：R733.71[医药卫生—肿瘤]