纤维素酶与淀粉酶降解壳聚糖的动力学研究  

ENZYME KINETICS OF AMYLASE AND CELLULASE ON HYDROLYZING CHITOSAN

作　　者：周桂[1] 何子平[2] 邓光辉[1] 黄在银[1] 谭学才[1] 

机构地区：[1]广西民族大学化学与生态工程学院,南宁530006 [2]广西农业科学院,南宁530006

出　　处：《海洋科学》2003年第11期59-62,67,共5页Marine Sciences

基　　金：广西壮族自治区教育厅资助项目

摘　　要：采用还原糖测定法 ,研究了纤维素酶与淀粉酶降解壳聚糖过程中酶反应动力学参数 ,比较了以壳聚糖为底物的纤维素酶与淀粉酶的催化反应动力学特性。研究结果表明 ,反应最适温度均为50℃ ;最适pH值为5.4 ;纤维素酶与淀粉酶降解壳聚糖米氏常数Km 值分别为 :1.68mg/mL、0.17mg/mL;最大酶反应速度Vmax分别为 :1.24mg·L-1·min-1、1.22mg·L -1·min -1。The enzyme kinetics of amylase and cellulase on hydrolyzing chitosan were studied in detail in this paper by investigating the effects of reaction temperature, pH, time, substrate and enzyme concentration on the hydrolytic rate. Both enzymes had the same optimum temperature of 50 ℃ and similar optimum pH of 5.4. The Km values of amylase and cellulase were 1.68 mg/mL and 0.17 mg/mL, and their maximum reaction velocity, i.e. Vmax, were 1.24 mg·L-1·min-1 and 1.22 mg·L-1·min-1 respectively.

关 键 词：壳聚糖降解 纤维素酶 淀粉酶 酶反应速度 

分 类 号：Q55[生物学—生物化学]