舒林酸对结肠癌变中Fas和FasL蛋白表达影响的初步研究  

Preliminary Study on Effect of Sulindac on Fas & FasL Protein Expression in ColonicTumorigenesis

作　　者：孙保存[1] 赵秀兰[1] 阎雪燕[2] 刘易欣[1] 王欣[1] 张诗武[3] 孙燕[1] 

机构地区：[1]天津医科大学病理教研室,天津医科大学肿瘤医院300070 [2]天津医科大学总医院消化科 [3]天津医科大学肿瘤医院

出　　处：《天津医药》2003年第11期716-719,共4页Tianjin Medical Journal

基　　金：天津市自然科学基金资助课题 (项目编号 :973611311)

摘　　要：目的 :探讨舒林酸诱导肿瘤细胞凋亡可能存在的分子机制。方法 :利用二甲肼诱发的小鼠实验性大肠癌动物模型 ,采用免疫组织化学染色的方法 ,分别标记Fas及FasL阳性细胞 ,观察诱癌过程中Fas和FasL蛋白表达细胞的分布与密度变化和舒林酸对它们的影响。结果 :Fas和FasL的表达阳性产物主要位于细胞浆 ,阳性细胞均位于大肠黏膜表层上皮中 ,呈簇状分布 ,舒林酸预防组和治疗组Fas和FasL表达量与模型组比较差别有统计学意义 (P<0.05)。结论 :舒林酸可以上调Fas和FasL的表达 ,诱导细胞凋亡 ,抑制肿瘤形成和发展。Objective:To preliminarily study the effect of sulindac on Fas & FasL protein expression in colonic tumorigenesis.Methods:In the 1,2-dimethylhydrazin(DMH)-induced colorectal animal models,Fas & FasL expression was detected by immunohistochemical staining in order to observe the distribution of the cells expressing Fas & FasL and the effect of sulindac on them in different groups.Results:The positive products of Fas & FasL were located in cytoplasm.The positive cells were all in the surface epithelial cells of colorectal mucosa and disposed into clusters.The Fas & FasL expression in preventive and treatment groups was higher than that in model group(P<0.05).Conclusion:Sulidac can induce apoptosis by up-regulating the expression of Fas & FasL and inhibit the development of colon cancer.

关 键 词：舒林酸 结肠癌 FAS蛋白 FASL蛋白 癌细胞凋亡 动物模型 

分 类 号：R735.35[医药卫生—肿瘤] R730.53[医药卫生—临床医学]