幽门螺杆菌感染诱发蒙古沙鼠胃黏膜肠上皮化生和异型增生  

INDUCTION OF INTESTINAL METAPLASIA AND DYSPLASIA IN MONGOLIAN GERBILS INFECTED WITH HELICOBACTER PYLORI

作　　者：兰春慧[1] 张渊智[1] 房殿春[1] 

机构地区：[1]第三军医大学西南医院全军消化专科中心

出　　处：《解放军医学杂志》2003年第11期985-986,990,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金 (编号 39870 345);全军"十五"重点基金(编号 0 1Z0 75)资助课题

摘　　要：目的　通过建立幽门螺杆菌 (H pylori)感染蒙古沙鼠的动物模型 ,观察H pylori及H pylori与N 甲基 N’ 硝基 N 亚甲基胍 (MNNG)联用作用后胃黏膜的组织学改变 ,并观察了维生素C的预防作用。方法　 16 0只SPF级蒙古沙鼠随机分为 5组 ,每组 32只。A组单用H pylori菌液灌胃 ;B组在接种H pylori后 4周 ,摄入MNNG水 (2 0 μg/ml) ,连续 30周 ;C组单用MNNG水 (2 0 μg/ml) ,连续 30周 ;D组在B组基础上同时摄入加维生素C的食料 ;E组为空白对照组。各组分别在实验后 12、36、4 8、5 2周各处死 8只 ,取胃黏膜行组织学检查 ,用Warthin Starry银染、PCR和快速尿素酶法检测H pylori。 结果　累计至 5 2周肠化生和异型增生的发生率A组(87 5 % ,6 2 5 % )、B组 (78 1% ,5 6 3% )显著高于C组 (6 2 % ,6 3% )、D组 (15 6 % ,9 4 % )和E组 (0 % ,0 % ) ,P <0 0 1。B组的H pylori感染率从接种后 12周的 10 0 %下降到 5 2周的 6 6 7%。结论　H pylori感染与胃癌发生有关 。Objective Using a model of H.pylori infected Mongolian gerbil , we observed the effect of H.pylori and N methyl N’ nitro N nitrosoguanidine (MNNG) on gastric mucosa, in an attempt to clarify the potential role of vitamin C in the prevention of gastric carcinoma. Methods A total of 160 Mongolian gerbils , eight week old, were randomly divided into five groups(each 32 animals): Group A, infected with H.pylori ; Group B, infected with H.pylori followed by MNNG administration; Group C, received MNNG without H.pylori infection; Group D, infected with H pylori followed by administration of MNNG and vitamin C; Group E as control. Eight animals from each group were killed at 12, 24, 36, 48 weeks, and histopathological changes in their stomachs were examined for chronic gastritis, intestinal metaplasia, atypical hyperplasia and adenoma. Results The incidences of intestinal metaplasia and dysplasia in groups A and B were significantly higher than those in the other groups( P <0 01). The incidence of H pylori infection was decreased from 100% at 12 weeks to 66 7% at 52 weeks in group B. Conclusion H pylori infection is related with gastric carcinogenesis, and vitamin C may prevent the development of gastric carcinoma

关 键 词：幽门螺杆菌 胃肿瘤 化学预防 N-甲基-N'-硝基-N-亚甲基胍 维生素C 

分 类 号：R573.3[医药卫生—消化系统]