欧芹素乙和已酮可可碱对白细胞介素-1_β诱导脑血管内皮细胞与单核细胞粘附和迁移的抑制作用  

INHIBITORY EFFECTS OF IMPERATORIN AND PENTOXIFYLLINE ON MONOCYTE ADHESION AND TRANSMIGRATION TO BRAIN MICROVASCULAR ENDOTHELIAL CELLS INDUCED BY INTERLEUKIN-1 BETA

作　　者：储智勇[1] 芮耀诚[1] 张蕾[1] 马金刚[1] 

机构地区：[1]中国人民解放军第二军医大学药学院药理教研室,上海200433

出　　处：《解放军药学学报》1999年第2期1-3,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：军队"九五"科研项目

摘　　要：目的：研究白细胞介素－１β（ＩＬ－ １β） 对培养的牛脑微血管内皮细胞（ＢＣＭＥＣ） 与单核细胞（ＭＮＣ） 粘附、迁移的影响及药物的保护作用。方法：通过测定粘附于内皮细胞单层上的ＭＮＣ及胶原层中的ＭＮＣ，计算ＭＮＣ的粘附率及迁移率。结果：ＩＬ－ １β（５００ｋＵ·Ｌ－ １） 可促进单核细胞与脑微血管内皮细胞的粘附与迁移，其促粘附与迁移作用具明显的时效关系。ＩＬ－ １β促粘附达最大效应时间为２ｈ，较对照组提高４６．１％ ；促迁移作用２ｈ 达最大值，较对照提高１１８ ．１ ％ 。欧芹素乙、己酮可可碱在浓度１ ～１００μｍｏｌ·Ｌ－１ 范围时，可剂量依赖性地拮抗ＩＬ－ １β诱导的粘附及迁移，药物浓度最高（１００μｍｏｌ·Ｌ－１） 时，抑制率达最大。AIM To study inhibitory effect of imperatorin(IMP) and pentoxifylline(PTX) on monocyte adhesion and transmigration to bovine brain microvascular endothelial cell (BCMEC) induced by interleukin-1β(IL-1β).METHODS The adhesion and migration of bovine monocyte were measured using a model system comprising BCMEC cultured on collagen gels in vitro. RESULTS Preincubation of BCMEC layers with IL-1β(500 KU·L -1 ) enhanced monocyte adhesion and transmigration.Both adhesion and transmigration of monocytes were time-dependently increased.After 2h incubation of BCMEC with IL-1β,adhesion or migration reached maximum (33.7% and 17.4% respectively).At the concentrations from 1 to 100μmol·L -1 inhibited the monocyte adhesion and migration in a concentration-dependent manner,at 100μmol·L -1 the maximal adhesion inhibitory effects were 85.4% and 78.8% and their maximal migration inhibitory effects were 72.3% and 70.3%,respectively.CONCLUSION IMP and PTX decrease monocyte adhesion and transmigration to BCMEC induced by IL-1β.

关 键 词：欧芹素乙 已酮可可碱 白细胞介素-lβ 脑血管 内皮细胞 单核细胞 抑制作用 动脉粥样硬化 AS 

分 类 号：R743[医药卫生—神经病学与精神病学] R97[医药卫生—临床医学]