电泳法测定基因工程蛋白分子量和纯度及方法比较  

Determination of Molecular Weight of Genetic Engineering Protein by Level and Vertical Electrophoresis

作　　者：叶子坚[1] 姚善泾[2] 

机构地区：[1]江西萍乡高等专科学校化工系,萍乡337055 [2]浙江大学化工学院生物化工系,杭州310027

出　　处：《井冈山医专学报》1999年第2期18-21,共4页Journal of Jinggangshan Medical College

基　　金：国家自然科学基金

摘　　要：目的：为基因蛋白含量的分析寻找更为快速、有效的实验室方法。方法：SDS-水平电泳法、SDS-垂直电泳法。结果：SDS-水平电泳法的分辨率较差；SDS-垂直电泳法的分辨率较高。结论：水平电泳法和垂直电泳法都可用于目标蛋白质分子董确定和纯度的鉴定，但后者明显优于前者。Objective: To seek for a more efficient and quicker experimental way to analyse thecontent of gene amumen. Methods: SDS- horizontal electrophoresis; SDS - vertical electrophoresisResults: The resolving power of the SDS - horizontal electrophoresis is stronger than that of the SDS -vertical electrophoresis. Conclusions: Both SDS - horizontal eletrophoresis and SDS - venical elec-trophoresis can be used to confirm the molecular weight of the target protein and to examine its purity.The latter method is better than the former one.

关 键 词：电泳法 基因工程 分子量 生长因子 蛋白质 

分 类 号：Q78[生物学—分子生物学]