针刺足三里穴对大鼠下丘脑催产素、加压素mRNA表达的影响  

Effect of oxytocin and vasopressin mRNA expression in hypothalamus by acupuncturing acupoint ST36

作　　者：孙锦平[1] 田庆华[1] 尹岭[1] 母义明[1] 朱克[1] 

机构地区：[1]解放军总医院神经信息中心,北京市100853

出　　处：《中国康复理论与实践》2003年第11期650-651,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(No30171135);国家科技部基础研究重大项目前期研究专项(2001CCA00700)

摘　　要：目的研究针刺足三里穴对下丘脑催产素、加压素mRNA的表达在不同时间点的特异性影响。方法将 78只SD雄性大鼠分为针刺足三里穴位组、针刺足三里穴位旁开点组、束缚组、正常对照组 ,应用RT PCR方法观察针刺后 2、4、6、8h的下丘脑催产素、加压素的表达情况 ,经图像分析系统照相并进行半定量分析。结果针刺穴位旁开点后 2、4h下丘脑催产素的表达与其他 3组相比具有显著差异 (P <0 0 5 ) ,针刺后 6、8h各组间相比无显著性差异 (P >0 0 5 )。针刺后 2、4、6、8h ,针刺足三里穴位组的加压素表达较其他 3组均降低 ,有显著性差异 (P <0 0 5 )。结论针刺穴位旁开点可以引起催产素短时间的表达增高 ,而穴位点刺激并不引起催产素的表达变化。针刺足三里穴位可以引起加压素长时间表达受抑制 ,针刺穴位旁开点对下丘脑加压素的表达无影响。ObjectiveTo study the special effect of oxytocin and vasopressin mRNA expression by acupuncturing acupoint ST36.Methods78 SD male rats were divided into four groups, acupuncturing ST36 group, acupuncturing para-acupoint ST36 group, bondage group and control group. RT-PCR was used to observe the oxytocin and vasopressin mRNA expression in 2,4,6,8 hours after acupuncture, semi-quantitative result was obtained by using image analysis system.ResultsOxytocin mRNA expression in hypothalamus in 2,4 hours was the statistically significant different in acupuncturing para-acupoint ST36 group contrast with other three groups, while there was not statistically different between all groups on oxytocin mRNA expression in 6,8 hours. Vasopressin mRNA expression in hypothalamus in 2,4,6,8 hours was statistically depressed in acupuncturing acupoint ST36 contrast with other three groups.ConclusionOxytocin mRNA expression in hypothalamus increases for short time after acupuncturing para-acupoint ST36. Vasopressin mRNA expression in hypothalamus decreases for long time after acupuncturing acupoint ST36.

关 键 词：足三里 催产素 加压素 下丘脑 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学] R392.1[医药卫生—临床医学]