电针大鼠足三里穴对脑干P物质基因表达的影响  

Effects of electroacupuncture of Zusanli on substance P mRNA expression in rat brain stem

作　　者：田庆华[1] 孙锦平[1] 尹岭[1] 母义明[1] 

机构地区：[1]解放军总医院神经信息中心,北京市100853

出　　处：《中国康复理论与实践》2003年第11期652-653,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(30171135);国家科技部基础研究重大项目前期研究专项(2001CCA00700)

摘　　要：目的观察电针足三里对大鼠脑干P物质 (SP)基因表达的影响及其规律 ,探讨脑肠肽参与针刺镇痛以及治疗胃肠疾病的机制。方法将 78只SD大鼠随机分成正常对照组以及针刺足三里、非经非穴以及束缚后各 2h、4h、6h、8h组。应用RT PCR方法结合计算机图像分析测定脑干SP的基因表达。结果正常情况下脑干内有一定的SPmRNA表达 ,足三里组、非经非穴组在电针 2h后脑干SP的基因表达增加 (P <0 .0 5 ) ,6— 8hSPmRNA的表达两组间有显著性差异 (P <0 .0 5 )。结论电针可以增加大鼠脑干SP的基因表达 ,而在不同的时间点电针足三里穴与非经非穴点SP基因表达的不同说明了针刺穴位点的作用 ,这可能是电针足三里穴SP参与针刺镇痛和调节胃肠运动的机理之一。ObjectiveTo investigate effects of electroacupuncture(EA) of Zusanli(ST36) on changes of substance P(SP) mRNA expression in rat brain stem.Methods78 SD rats were evenly randomized into Zuanli-EA group, non-acupoint group, bondage groups(at the end of 2h,4h,6h,8h after 30 minute EA stimulation in each group) and control group. RT-PCR and image processing were used to study the change of SP mRNA level in brain stem.ResultsIn control groups,there was SP expression. An increase of SP mRNA expression was seen in Zusanli group and non-acupoint group comparing with control group at the end of 2h after 30min EA stimulation(P<0.05).There was difference between above two groups at the end of 6h and 8h(P<0.05).ConclusionEA stimulation elicited an accelerated expression of SP gene. Zusanli-EA stimulation can increased this expression, which may constitute one of the mechanisms for gastrointestinal motility and eletroacupuncture analgesia.

关 键 词：电针 足三里 P物质 基因表达 脑干 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学] R392.1[医药卫生—临床医学]