锌对体外培养小鼠胎鼠前肢骨发育的影响  

Effects of zinc on bone development in mouse embryo in vitro

作　　者：余增丽[1] 李云[1] 郑艾[2] 

机构地区：[1]华西医科大学公共卫生学院营养与食品卫生教研室,成都610041 [2]华西医科大学第二附属医院

出　　处：《中华预防医学杂志》2002年第2期97-99,F004,共4页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助 (3 960 0 12 2 )

摘　　要：目的　研究锌对骨发育的影响。方法　用一次通气旋转装置 ,在缺锌的基础培养基和分别加入 4 5、70和 12 0 μmol/LZn2 + 的培养基中对 16d孕龄胎鼠前肢进行培养。结果　培养基中缺锌和在培养基中补充Zn2 + 至Zn2 + 浓度 12 0 μmol/L时 ,骨组织中骨钙素 (OC)和45Ca含量减少 ,碱性磷酸酶 (AKP)活性显著降低 ;当在培养基中补充Zn2 + 至Zn2 + 浓度 4 5、70 μmol/L时OC合成量、骨组织对钙的吸收及AKP活性显著增加。培养液中缺锌及锌浓度为 12 0 μmol/L时 ,X线显示 ,长骨长度和密度与其自身对照相比 ,长骨长度较短 ,骨密度降低 ;当培养液中Zn2 + 浓度分别为 4 5和 70 μmol/L时 ,与其自身对照相比 ,长骨长度及其骨密度有所增加。组织学分析显示 ,培养液中缺锌及锌浓度为 12 0 μmol/L时 ,镜下可见骨细胞死亡 ,基质丢失 ;当培养液中Zn2 + 浓度分别为 4 5和 70 μmol/L时 ,骨细胞增殖、分化活跃 ,类骨质的分泌与合成增加。结论　适量补锌能促进骨组织形成及发育 ,锌缺乏或过量时均可影响骨组织正常生长发育及代谢。Objective This study was designed to explore the effects of zinc on bone development Methods Forelimbs of mice with 16 d gestation were cultured by a rotating device Results Contents of OC and 45 Ca and activities of AKP in the bone tissues cultured at zinc deficiency media and at media with 120 μmol/L of Zn 2+ decreased significantly Synthesis of OC, absorption of calcium and activities of AKP in the bone tissues cultures at media with 45 and 70 μmol/L of Zn 2+ increased significantly Radiograph of bone tissues showed that length of long bone cultured at zinc deficiency media and at media with 120 μmol/L of Zn 2+ shortened and their density reduced, and those cultured at media with 45和70 μmol/L of zinc increased, as compared with self control bone Histological analysis showed the death of bone cells and loss of stroma in the bone tissues cultured at media with 120 μmol/L of Zn 2+ , and active proliferation and differentiation of bone cells, and secretion and synthesis of osteoid increased in the bone tissues cultured at media with 45和70 μmol/L of zinc Conclusions Adequate supplementation of zinc could promote formation and development of bone tissues and deficiency or excess of zinc could alter their growth and development and normal metabolism

关 键 词：锌 体外培养 小鼠 胎鼠 前肢骨 骨发育 

分 类 号：R151.3[医药卫生—营养与食品卫生学]