Smac对骨肉瘤细胞系Saos-2生长及耐药性的影响  

Effects of Smac on growth and drug resistance of human osteosarcoma Saos-2 cells

作　　者：金军[1] 赵健[1] 张霞[1] 张瑞萍[1] 石梅[1] 李晓东[1] 吕岩[1] 郭亚军[1] 

机构地区：[1]第二军医大学国际合作肿瘤研究所,上海200433

出　　处：《第二军医大学学报》2003年第11期1225-1227,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30000008)

摘　　要：目的:观察Smac表达对骨肉瘤细胞Saos-2生长和耐药性的影响。方法:构建smac和反义smac表达载体pcDNA-smac 和pcDNA-anti-smac,G418筛选出分别稳定转染pcDNA-smac和pcDNA-anti-smac的骨肉瘤细胞Saos-2。利用流式细胞仪检测各转染细胞的细胞周期分布;绘制各细胞在6 d内的生长曲线以观察各细胞生长情况的变化。同时采用MTT法检测各转染Saos-2细胞对依托泊苷的敏感性。结果:与转染空载体pcDNA3.0的Saos-2细胞相比,转染反义smac的Saos-2细胞G1/G0期比例升高,细胞生长自第4天起明显加快。转染smac的Saos-2细胞的细胞周期及生长曲线无明显差别;依托泊苷作用细胞后,与转染空载体pcDNA3.0的Saos-2细胞相比,转染反义smac的Saos-2细胞的存活率增高;而转染smac的Saos-2细胞的存活率降低。结论:抑制Smac在细胞中表达,可使Saos-2细胞生长加快,增强对依托泊苷的耐受性,Smac在细胞中的过表达可提高Saos-2对依托泊苷的敏感性。Objective:To observe the effect of second mitochondria-derived activator of caspase(Smac) expression on the growth and drug resistance of the human osteosarcoma cell line Saos-2. Methods: The pcDNA-smac and pcDNA-anti-.smac vector were constructed and transfected into the Saos-2 cells. The pcDNA-smac, pcDNA-anti-smac and pcDNAS. 0 transfect-ed Saos-2 cells were obtained by G418 screening. The cell cycle of the transfecting Saos-2 tested by flow cytometry and the cell growth curves were mapped by cell counting. The sensitivity of Saos-2 cells to etoposide were detected by MTT method. Results: Compared with the control cells transfected with empty vector pcDNA3. 0, the transfecting percentage of the pcD-NA-anti-smac cell in the G|/G0 phase increased and its growth was accelerated. After treated with etoposide, the viability of the pcDNA-anti-smac transfected cell increased and the viability of the pcDNA-smac transfected cell decreased. There was little difference on the cell cycle and the cell growth between the pcDNA-smac transfected cells and the control cells. Conclusion: Inhibiting Smac expression in Saos-2 cells arrests the cell in G1/G0 and increases cell resistance to etoposide. Over-expression of Smac makes Saos-2 cell more sensitive to etoposide.

关 键 词：SMAC 骨肉瘤 细胞系 SAOS-2细胞 细胞生长 耐药性 

分 类 号：R738.1[医药卫生—肿瘤]