原位杂交检测尸检组织中SARS-CoV RNA  

Detection of severe acute respiratory syndrome(SARS)-associated coronavirus RNA in autopsy tissues with in situ hybridization

作　　者：张庆玲[1] 丁彦青[1] 候金林[2] 贺莉[1] 黄仲曦[1] 王慧君[1] 蔡俊杰[1] 张进华[1] 张文丽[1] 耿舰[1] 李欣[1] 康伟[1] 杨磊[1] 申洪[1] 李祖国[1] 韩惠霞[1] 陆药丹[1] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515 [2]第一军医大学南方医院传染科,广东广州510515

出　　处：《第一军医大学学报》2003年第11期1125-1127,共3页Journal of First Military Medical University

基　　金：国家自然科学基金SARS紧急启动项目(30340015);军队医药卫生SARS专项(03F016-2);广东省SARS专项(GD2003-80)~~

摘　　要：目的从分子水平检测急性重症呼吸综合征(SARS)患者的病变组织中SARS病毒(SARS-associated coronavirus, SARS-CoV)的存在和分布情况。方法应用原位杂交技术检测因SARS死亡患者的肺、脾脏、淋巴结、垂体、胰腺、甲状旁腺、肾上腺、胃肠道、皮肤、脑、肝、肾、血管、四肢横纹肌组织、骨髓、心脏、卵巢、子宫和睾丸等组织的SARS-CoV RNA的表达和定位。结果尸检组织多部位(包括肺泡上皮细胞、气管及支气管浆液腺上皮细胞、肺内单核/巨噬细胞、脾脏和淋巴结的单核/巨噬细胞、胰腺腺泡细胞、垂体嗜酸性细胞、肾上腺皮质细胞、甲状旁腺嗜酸性细胞、食道鳞状上皮、胃肠道上皮细胞及胃粘膜壁细胞、皮肤汗腺细胞、大脑神经元细胞、肝细胞、肾远曲小管上皮细胞、骨髓早幼粒细胞及小静脉内皮细胞)SARS-CoV RNA阳性。结论SARS-CoV可侵犯全身多种器官;SARS-CoV在机体的分布情况与冠状病毒受体CD13分布相似;皮肤汗腺、消化道上皮及肾远曲小管上皮细胞SARS-CoV RNA阳性对确定SARS传播途径具有重要意义。Objective To explore the distribution of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in SARS autopsy tissues at the molecular level. Methods In situ hybridization was used to detect the expression and location of SARS-CoV RNA polymerase gene in autopsy tissues from SARS-Cov-infected subjects, including the lung, spleen, lymph nodes, pituitary, pancreas, parathyroid, adrenal glands, gastrointestinal tract, skin, brain, liver, kidney, blood vessels, striated muscles of the limbs, bone marrow, heart, ovary, uterus and testicles. Result SARS-CoV RNA was detected in the cytoplasm of the alveolar epithelia, infiltrating mononuclear phagocytes in the lungs, serous gland epithelium of the trachea/bronchus, monocytes in the spleen and lymph nodes, acinar cells in the pancreas, acidophilic cells in the parathyroid and pituitary, adrenal cortical cells, epithelia of the alimentary tracts, gastric parietal cells, sweat gland cells, brain neurons, hepatocytes near the central vein, epithelia of the distal renal tubules, bone marrow promyelocytes, and endothelia of the small veins. Conclusions SARS-CoV invades various organs of the body and distributes in a similar fashion to CD13, the receptor of human coronavirus 229E. The detection of SARS-CoV in the sweat glands, alimentary tracts and epithelia of the distal convoluted tubules of the kidney may help identify the transmission routes of SARS-CoV.

关 键 词：原位杂交 检测 尸检组织 SARS-CoVRNA 急性重症呼吸综合征 SARS 

分 类 号：R361[医药卫生—病理学]