安徽省114株EHECO_(157)∶H_7菌株毒力因子基因的初步分析  

Primary Analysis of Virulent Genes of 114 Strains EHEC O_(157) ∶H_7 in Anhui Province

作　　者：胡万富[1] 贾嘉[1] 虞勇[1] 陆美娟[1] 

机构地区：[1]安徽省卫生防疫站,安徽合肥230061

出　　处：《疾病监测》2003年第10期367-369,共3页Disease Surveillance

摘　　要：目的　对安徽省近几年分离的肠出血性大肠杆菌O1 57∶H7(EHECO1 57∶H7)菌株的毒力因子基因进行实验室检测 ,以分析和研究肠出血性大肠杆菌O1 57∶H7的毒力特征和致病特性。方法　采用聚合酶链反应(PCR)检测EHECO1 57∶H7的两种重要的毒力因子志贺样毒素 (SLT)和溶血素 (hly)的特异性基因片段 ,并辅以血清学试验、生化试验进行菌株的生物学特性鉴定。结果和结论　检测结果显示 ,有 6 3 2 %的EHECO1 57∶H7菌株携带SLT、hly基因 ,动物源的菌株以家禽较高 ,家畜次之 ,3株源自腹泻病人的菌株毒力因子基因携带率为33 3% ,说明分离的EHECO1 57∶H7菌株具有较强的致病性 ;114株菌株中 ,有 14 0 %的菌株在 2 4h内发酵山梨醇 ,发酵山梨醇菌株的毒力因子基因携带率 18 8% ,显著低于不发酵菌株的 70 4 % 。To analyze and study the virulent Characteristic and the pathogenesis of EHEC O 157 ∶H 7; and to test the virulent genes of EHEC O 157 ∶H 7 isolated in Anhui Province. Methods Using PCR to test the specific gene fragment of two virulent factors (SLT and hly) of EHEC O 157 ∶H 7; and using serology test and biochemical test to identify to biological character of the strains. Result 63.2% of EHEC O 157 ∶H 7 strains carried SLT and hly genes, among the animal original strains livestock's given the highest rate, the rate of poultry was the second. It showed that the pathogenicity of EHEC O 157 ∶H 7 was powerful. 14.0% of the 114 isolated strains metabolized sorbitol in 24 hours, 18.8% of the strains that metabolism sorbitol carried these genes, which was lower than that did not metabolize sorbitol (70.4%), it showed some relevance between the mutation of metabolism sorbitol and lack of virulent genes. 〔

关 键 词：安徽 EHECO157:H7 毒力因子 基因缺失 基因携带率 肠出血性大肠杆菌 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]