口蹄疫3A蛋白基因的表达及其抗原性研究  

Studies on 3A Protein Gene Expression and Antigenetic Analysis of Foot and Mouth Disease Virus

作　　者：龚振华[1] 蒋正军[1] 刘俊辉[1] 孙淑芳[1] 王永玲[1] 曲志娜[1] 黄秀梅[1] 周清燕 陆明哲[1] 司红伟 王玉东[1] 郑增忍[1] 

机构地区：[1]农业部动物检疫所,青岛266032 [2]湖南省汀潭县畜牧水产局,411228 [3]青岛市畜牧局,266000

出　　处：《中国动物检疫》2003年第12期23-25,共3页China Animal Health Inspection

摘　　要：从pUCm-3ABC质粒切割FMDV的3A蛋白基因DNA,将其与pET-30c(+)载体连接,构建pET30C3A原核重组表达质粒,序列分析表明,pET30C3A重组质粒的插入3A蛋白基因的阅读框架正确。pET30C3A-BL21经IPTG诱导后可表达3A融合蛋白。Western Blot试验证明该表达蛋白具有较好的抗原活性,提示可进一步用重组表达的FMDV 3A蛋白作抗原检测PMD。The DNA coding for 3A protein of foot and mouth disease virus was cloned into pET- 30c(+) expression vector, and the reconstructed plasmid pET30C3A was obtained, which could express the 3A protein with high efficiency . Western Blot test showed that the expressed 3A protein could react specifically with FMDV positive serum,indicating that pET30C3A plasmid could be used for FMDV diagnosis.

关 键 词：口蹄疫 3A蛋白基因 基因表达 抗原性 

分 类 号：S852.65[农业科学—基础兽医学]