ANDROGEN REPRESSION OF CYTOKERATIN GENE EXPRESSION DURING RAT PROSTATE DIFFERENTIATION:EVIDENCE FOR AN EPITHELIAL STEM CELL-ASSOCIATED MARKER  

ANDROGEN REPRESSION OF CYTOKERATIN GENE EXPRESSION DURING RAT PROSTATE DIFFERENTIATION：EVIDENCE FOR AN EPITHELIAL STEM CELL－A

作　　者：王晓怀 Jer-TsongHsieh 

机构地区：[1]TheThirdMedicalDept.,OuaxlgzhouGeneralHospitalofPLA.Guangzhou510010. [2]DepartmentofUrology,TheUniversityofTexasMDAndersonCancerCenter,Houston,TX77030,U.S.A.

出　　处：《Chinese Medical Sciences Journal》1994年第4期237-241,共5页中国医学科学杂志（英文版）

摘　　要：Cytokeratin (CK) 8 mRNA expression in developing and degenerating rat prostate was studied using in situ hybridization with an antisense RNA-probe. It was found that : 1) the CK 8 antisense probe was accumulated only within prostatic epithelial cells ; 2) after castration, CK 8 mRNA signals in ventral prostute(VP) sections were significantly increased, and elevated CK 8 mRNA expression persisted even long after prostate involution was complete; and 3) during prostate development,the strongest CK 8 mRNA staining was found in the early neonatal prostatic epithelia which were composed mainly of prostatic stem cells. Thereafter, a shift of CK 8 mRNA staining to peripheral regions and decreased overall CK 8 mRNA levels were noted. These data indicate that excessive expression of CK 8 mRNA is a characteristic of prostatic stem cells, and CK molecules are excellent markers for determing the hierarchical pathway of cell differentiation in prostate epithelium.

关 键 词：prostate differentiation CYTOKERATIN in situ hybridization 

分 类 号：R394[医药卫生—医学遗传学]