慢性淋巴细胞白血病bcl-2基因表达与重排的研究(英文)  

The expression and rearrangement of bcl-2 gene in chronic lymphocytic leukemia

作　　者：向直富[1] 陈燕[1] 李慧玉[1] 李崇渔[1] 

机构地区：[1]同济医科大学附属协和医院血液病研究所,武汉430022

出　　处：《中国实验血液学杂志》1998年第1期45-48,共4页Journal of Experimental Hematology

摘　　要：人类恶性肿瘤常伴有非随机性染色体异常,并由于使调控细胞生长的基因表达失控而在肿瘤的发生中发挥十分关键的作用。bcl-2基因由于t(14,18)染色体易位而激活在低恶度的非霍奇金淋巴瘤的发生和演变中的作用已举世公认。类似的重排和非重排引起的bcl-2过度表达也见于慢性淋巴细胞白血病。我们应用免疫组化染色和聚合酶链反应检测了11例慢性淋巴细胞白血病bcl-2基因表达和重排,结果发现所有病例均高度表达Bcl-2蛋白,1例有t(14,18)(q32,q21)染色体易位。结论提示慢性淋巴细胞白血病普遍存在bcl-2基因高表达,bcl-2基因在慢性淋巴细胞白血病的发生和发展中发挥着十分重要的作用。Nonrandom chromosome aberrations are associated with human malignancies and belived to play a key role in the pathogenesis of these diseases by disturbing cellular genes involved in the control of cell growth, bcl-2 Gene becomes transcriptionally deregulated in the majority of low-grade non-Hodgkin's lymphomas as a result of t( 14, 18) translations that place the bcl-2 gene at 18q21 into juxtaposition with the Ig heavy-chain locus at 14q32. This rearrangement and non-rearranged overexpression of bcl-2 gene have been reported to occur in some cases of B-cell chronic lymphocytic leukemia(B-CLL). By using immunohistochemical staining method and polymerase chain reaction (PCR) analysis, the expression and rearrangement of bcl-2 gene in 11 cases of chronic lymphocytic leukemia(CLL) were detected. Overexpression of bcl-2 gene was found in all cases; 1 of 11 cases had t(14,18) (q32, q21) translations. The data indicated that high levels of bcl-2 gene expression occur frequently in CLL, and bcl-2 gene plays a pivotal role in pathogenesis and progression of CLL.

关 键 词：慢性淋巴细胞白血病 BCL-2基因 基因重排 基因表达 

分 类 号：R733.72[医药卫生—肿瘤]