用分子细胞遗传学技术检测白血病染色体易位  

Detection of chromosomal translocations of human leukemias using molecular and cytogenetic techniques

作　　者：王林[1] 牛超[1] 苏欣莹[1] 何凯莉[1] 曹琪[1] 陈竺[1] 陈赛娟[1] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海血液学研究所,上海200025

出　　处：《中国实验血液学杂志》1998年第2期85-90,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金;Dr.Clyde Wu基金

摘　　要：本文概述了荧光素原位杂交(FISH),染色体涂抹(CP)和比较基因组杂交(CGH)等FISH相关技术,并讨论了这些技术应用于多种染色体易位的白血病的检测,其中包括对急性早幼粒细胞白血病(APL),儿童B系急性淋巴细胞白血病(B-ALL)及慢性粒细胞白血病急性变(CML-BT)病人的染色体易位的检测。这些技术的应用为白血病发病的分子机制的阐明奠定了基础,同时为临床诊断和残余白血病细胞监测提供了直观、快速、敏感和特异的方法。This report summarizes the fluorescence in situ hybridization (FISH) and FISH-associated techniques such as chromosome painting (CP) and comparative genomic hybridization (CGH), and describes their application for detection of several chromosomal translocations of human leukemias-APL, ALL, and CML-BT. The results of using these techniques indicated that FISH and FISH-related techniques give the clue to study the molecular mechanism of leukemogenesis and provide a rapid, sensitive and specific method for clinical diagnosis and detection of residual leukemia celis.

关 键 词：荧光素原位杂交 染色体涂抹 比较基因组杂交 复合型染色体易位 急性淋巴细胞白血病 慢性粒细胞白血病急性变 急性早幼粒细胞白血病 

分 类 号：Q343.2[生物学—遗传学] R733.7[医药卫生—肿瘤]