核质互作型水稻线粒体不育基因的RAPD标记筛选  

Screening of RAPD Markers for Sterility Gene of Nucleo-cytoplasmic Interaction in Mitochondrial DNA of Rice

作　　者：裴得胜[1] 蔡平钟[1] 李名扬[2] 向跃武[1] 阎文昭[1] 张志雄[1] 侯磊[2] 张志勇[1] 裴炎[2] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]西南农业大学生物技术中心,重庆400716

出　　处：《作物学报》2003年第6期899-902,共4页Acta Agronomica Sinica

基　　金：国家 8 63计划项目 (2 0 0 1AA2 410 112 );四川省十五育种攻关项目资助

摘　　要：通过单因素的改变对核质互作型水稻线粒体DNA的RAPD反应条件进行优化 ,并在最佳条件下用OPERON公司OPA～OPM系列共 2 6 0个随机引物筛选水稻Ⅱ 32不育系、保持系、杂种一代线粒体DNA的差异片段 ,结果共获得 2 2个差异带。其中OPH19 180 0、OPJ0 9 4 0 0、OPJ18 14 0 0、OPJ18 10 0 0、OPJ2 0 30 0这几个特异片段可能与水稻雄性不育相关。我们正在用分子检测确定这些标记与细胞质雄性不育的关系。The reaction conditions of RAPD for mitochondrial DNA of nucleo-cytoplasmic interaction rice were optimized by changing a single factor. Under the optimum conditions, Ⅱ-32 male sterility line, maintainer line and F 1 hybrid mitochondrial DNA were screened by 260 RAPD primers from OPA to OPM. As a result, 22 differential fragments were found. Among them, OPH19-1800, OPJ09-400, OPJ18-1400, OPJ18-1000 and OPJ20-300 might have relation with rice male sterility. For further study, the relationship between the markers and cytoplasmic male sterility would be studied.

关 键 词：核质 互作型水稻 线粒体 不育基因 RAPD标记 基因筛选 

分 类 号：S511[农业科学—作物学]