肝片吸虫组织蛋白酶L1基因cDNA序列的克隆与分析  

Cloning and Sequencing of Cathepsin L1(FheCL1) Gene cDNA of Fasciola hepatica

作　　者：张韧[1] 李海云[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第5期282-285,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然基金项目 (No.3990 0 1 0 9);广东省自然科学基金项目 (No.980 1 2 8)~~

摘　　要：目的　研制肝片吸虫的候选DNA疫苗。　方法　根据国际发表的相关序列设计引物 ,在引物端加酶切位点 ,应用RT PCR方法。　结果　成功地将肝片吸虫组织蛋白酶L1(FheCL1)基因cDNA序列克隆进真核表达载体 pcD NA3 .1中。对所克隆的FheCL1基因序列及其推导的氨基酸序列进行分析 ,发现其与已发表序列相比 ,核苷酸序列有4.3 %的差异 ;推导的氨基酸序列有 6.9%的差异。两者蛋白质二级结构主要有 3个区域的区别 ,磷酸化位点有 10个不同 ,但共有一由 2 0个疏水氨基酸残基组成的保守区域。　结论　实验构建的 pcDNA3 .1 FheCL1重组质粒是一种新型的抗肝片吸虫DNA疫苗候选重组质粒 ;肝片吸虫可能存在不同的亚种 ,两者的FheCL1基因编码的第 1～ 2 0个氨基酸残基可能组成膜螺旋。Objective To search for a candidate DNA vaccine of Fasciola hepatica. Methods Using RT-PCR and digestion with Hind III and BamHI, Fasciola hepatica secreted cathepsin L1(FheCL1) cDNA was cloned into the expression vector pcDNA3.1. Results The cloning was successful, the cDNA sequence and its deduced amino acid sequence were analyzed. There was much difference between the cloned FheCL1 and the published one. But the first 20 residues of their amino acid sequences were the same. Conclusion The recombinant plasmid pcDNA3.1-FheCL1 may be a new type of candidate DNA vaccine candidate for Fasciola hepatica. It is possible that Fasciola hepatica presents different sub-species but their amino acid residues (1 to 20) encoded by FheCL1 might build up membrane spanning helix.

关 键 词：肝片吸虫 组织蛋白酶 L1基因 CDNA序列 克隆 

分 类 号：R383.26[医药卫生—医学寄生虫学]