大鼠体外循环动物模型的建立  

Establishment of Cardiopulmonary Bypass Model in Rats

作　　者：杨波[1] 苏肇伉[1] 陈恩[1] 

机构地区：[1]上海第二医科大学新华医院上海儿童医学中心小儿心胸外科,上海200092

出　　处：《上海第二医科大学学报》2003年第B10期12-14,42,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的 探讨建立大鼠体外循环动物模型的可行性。方法 以SD大鼠为研究对象,尾动脉插管灌注,右颈静脉插管引流,建立常温大鼠体外循环模型。转流1h,比较体外循环大鼠与对照大鼠的生理学指标。结果 现有条件下能够建立常温大鼠体外循环模型,较高流量(200ml·min^(-1)·kg^(-1))转流1h,转流期间保持存活,但容易出现代谢性酸中毒和动脉血压降低;转流结束后因血液过度稀释不能够长期存活。结论 无血预充常温大鼠体外循环模型可以成功建立,但是不能长期存活。Objective To determine the feasibility of establishing the cardiopulmonary bypass ( CPB) model in rats. Methods The tail artery serving as the arterial inflow and the right jugular vein as the venous drainage for the CPB circuit, we established the normothermic CPB model in SD rats. During the process of CPB for 1h, the physiological parameters were analyzed and compared with those of the controls. Results It is feasible to establish the normothermic CPB model in SD rats. The rats remained alive during CPB with a high flow rate (200ml·min-1·kg-1). The rats were prone to suffer metabolic acidosis during CPB and could not survive for a long time after CPB because of severe haemodilution. Conclusion It is feasible to establish the normothermic CPB model in rats without blood primes, however, the rats could not live for a long time.

关 键 词：大鼠 体外循环 动物模型 可行性 尾动脉插管灌注 

分 类 号：R-332[医药卫生]