脑胶质瘤bFGF蛋白表达与增殖活性的研究  

Relationship between bFGF expression and cellular proliferation in human glioma

作　　者：黄乔春[1] 陈谦学[1] 郑虎[1] 叶应湖[1] 阮航[1] 邵步云[1] 

机构地区：[1]武汉大学人民医院神经外科,430060

出　　处：《肿瘤防治研究》2003年第6期464-465,共2页Cancer Research on Prevention and Treatment

摘　　要：目的　探讨胶质瘤中碱性成纤维生长因子 (basicfibroblastgrowthfactor,bFGF)蛋白与增殖细胞核抗原 (proliferativecellnuclearantigen ,PCNA)蛋白在胶质瘤中的表达及相互关系。 方法　根据WHO标准进行分类 ,采用免疫组化方法 ,对 35例胶质瘤和 7例正常脑组织石蜡标本中bFGF与PCNA蛋白的表达水平进行检测 ,并比较二者的相关性。结果　bFGF和PCNA在高级别胶质瘤中表达较多 ,在低级别胶质瘤中表达较少 ,在正常脑组织中几乎不表达 ,两两比较差异有显著性 (P <0 .0 1) ,且二者呈显著正相关 (r=0 .74 ,P <0 .0 1)。结论　人脑胶质瘤bFGF蛋白的阳性表达与细胞增殖活性和组织学分级密切相关 ,它可能参与了人脑胶质瘤细胞的恶性转化。Objective To study the relationship between the expression of basic fibroblast growth factor (bFGF) and proliferative cell nuclear antigen (PCNA) in human glioma.Methods The expression levels of bFGF and PCNA in 35 cases of human glioma and 7 cases of normal brain tissue specimen were detected by immunohistochemical technique. Furthermore, the correlation between the expression of the bFGF and PCNA was compared.Results In normal brain tissue, group Ⅰ,Ⅱ and group Ⅲ,Ⅳ,the positive expression of bFGF was 9.29± 2.69, 23.85± 7.76 and 42.53± 12.37 respectively, and PCNA was 3.86± 1.07, 24.15± 6.12 and 57.07± 17.26 respectively. The difference were significant (P< 0.01)among them.Conclusion There is a positive correlation between the expression of the bFGF and cellular proliferation and histological grade of human brain gliomas. These findings suggested that bFGF plays an important role during the malignant behaviors of glioma.

关 键 词：脑胶质瘤 BFGF蛋白 表达 增殖活性 

分 类 号：R739.41[医药卫生—肿瘤]