小型猪腮腺放射损伤唾液流率和血常规及生化动态观察  

EFFECT OF IRRADIATION TO PAROTID GLAND OF MINIATURE PIG ON THE PAROTID FLOW RATE,BLOOD ROUTINE TEST AND SERUM CHEMISTRY

作　　者：单兆臣[1] 王松灵[1] 李钧[1] 祁森荣[1] 张春梅[1] 

机构地区：[1]首都医科大学口腔医学院涎腺疾病中心及基因治疗分子生物学实验室,首都医科大学博士研究生100050

出　　处：《北京口腔医学》2003年第4期191-194,共4页Beijing Journal of Stomatology

基　　金：国家杰出青年科学基金 (No .30 1 2 5 0 4 2 );国家自然科学基金 (No .30 2 71 4 0 0 )资助

摘　　要：目的 :动态观察小型猪腮腺放射损伤前后腮腺流率、血常规及血生化变化 ,为建立小型猪腮腺放射损伤动物模型 ,及基因治疗涎腺放射损伤奠定基础。方法 :试验组 8只小型猪 ,1 5Gy单侧腮腺放射损伤 ,对侧腮腺做对照 ,2只 0Gy放射做空白对照。放疗前、放疗后 4周、8周和 1 6周 ,用吸盘法收集唾液 ,前腔静脉取血 ,观察唾液流率、血常规和血生化改变。结果 :放疗后 8周唾液流率下降 ,1 6周唾液流率下降明显并与放疗前有显著性差别。随着放射后的时间延长 ,白细胞计数下降越来越明显 ,第 8周和 1 6周与放疗前有显著性差别。红细胞在放疗后第 8周及第 1 6周稍有增加但没有显著性差别 ,血小板在 4周下降 ,第 8周及第 1 6周增高超出放射前水平。乳酸脱氢酶血清含量放疗后呈持续下降趋势 ,与放疗前有显著性差别(P <0 0 5 )。血清淀粉酶在放疗后 1周增高 ,第 4周显著下降 ,与放疗前相比差别有显著性 ,随后缓慢回升。结论 :1 5Gy放疗1 6周时放疗侧腮腺唾液流率明显下降 ,血液常规白细胞计数和血清乳酸脱氢酶。Objective:To study the trending changes of minipig parotid salivary flow rate,blood routine examination and serum chemistry after irradiation (IR) damage to parotid gland.Methods:Ten minipigs were divided into 2 groups.Unilaterial,single dose irradiation (15Gy) was delivered via a liner accelerator to one side of parotid glands of eight minipigs,blank control to two minipigs.Saliva collections were done in pro IR,IR+4weeks,IR+8weeks,IR+16weeks.Blood was drawn at the same timepoints.Results:At 8 weeks post IR,decreased parotid saliva flow rates was found in the irradiated side,there was significant difference between the flow rates of 16weeks post IR and pro IR (P<0 05).At post IR,WBC count decreased.There was significant difference of WBC count between at 8weeks,16weeks post IR and pro IR.Compared with pro IR,LDH of serum significantly decreased after irradiation.Conclusion:At 16weeks post IR,saliva flow rate significantly decreased in irradiated parotid gland (15Gy).Compared with pro IR,at post IR WBC count and LDH and AMY of serum significantly changed.

关 键 词：小型猪 腮腺 放射损伤 唾液流率 血常规 唾液流率 

分 类 号：R781.7[医药卫生—口腔医学]