组织因子对人类大肠癌培养细胞侵袭能力的影响  

Role of tissue factor in the invasive ability of cultured human colon carcinoma cells

作　　者：姚宏伟[1] 万远廉[1] 叶京明[1] 吴楠[1] 鞠晓明[1] 朱静[1] 

机构地区：[1]北京大学第一医院普通外科

出　　处：《北京大学学报（医学版）》2003年第6期634-638,共5页Journal of Peking University:Health Sciences

基　　金：教育部科学技术研究重点项目资助 (0 2 0 0 3)~~

摘　　要：目的 :分析组织因子表达对人类大肠癌细胞 (HT 2 9细胞 )体外侵袭能力的影响。方法 :利用构建有正义/反义组织因子cDNA(TFcDNA)的真核表达载体 pcDNA3.1 /Zeo ,以脂质体法转染HT 2 9细胞 ,经验证转染成功的HT 2 9细胞采用Western印迹分析检测组织因子的蛋白表达水平并进行基质胶 (Matrigel)侵袭实验观察细胞体外侵袭能力的变化。结果 :转染了正义TFcDNA的HT 2 9细胞的组织因子表达水平及侵袭能力较未转染的HT 2 9细胞升高 ;转染了反义TFcDNA的HT 2 9细胞的组织因子表达水平及侵袭能力较未转染的HT 2 9细胞相比降低。结论Objective :To analyze the role of tissue factor (TF) in the in vitro invasive ability of human colon carcinoma cells (HT 29). Methods: The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT 29 cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. In vitro Matrigel invasion assays were used to show the invasive ability of HT 29 cells with sense/antisense TFcDNA transfection. Results: HT 29 cells with sense TFcDNA transfection showed increased TF expression and invasive ability compared with the cells without transfection, but HT 29 cells with antisense TFcDNA transfection got the contrary change. Conclusion: TF can increase the invasive ability of HT 29 cells in vitro.

关 键 词：组织因子 人类 大肠癌 培养细胞 侵袭能力 肿瘤浸润 转染 

分 类 号：R735.34[医药卫生—肿瘤]