Survivin基因在血液肿瘤细胞株细胞的表达  

Expression of Survivin gene in hematological tumour cell lines

作　　者：薛军[1] 林茂芳[1] 

机构地区：[1]浙江大学医学院附属第一医院血液科,浙江杭州310003

出　　处：《实用肿瘤杂志》2003年第6期453-456,共4页Journal of Practical Oncology

摘　　要：目的　探讨 Survivin基因在血液肿瘤细胞株细胞的表达及其可能的机制。方法　应用 RT- PCR半定量法检测 Survivin基因 m RNA表达。结果　血液肿瘤细胞株和 K5 6 2 / ADM细胞株细胞均有 Survivin m RNA表达 ,且均高于疾病对照组 (P<0 .0 0 1)。不同的血液肿瘤细胞株 Survivin m RNA表达有明显的差异性 ,Dami与K5 6 2、HL - 6 0、Jurkat、MU TZ- 1、Raji、6 T- CEM;NB4与 U 937、HEL、6 T- CEM、Jurkat、Dami;HEL与 MU TZ- 1之间Survivin m RNA的表达差异有显著性 (P值分别 <0 .0 0 1～ 0 .0 5 )。 K5 6 2 / ADM细胞株与 K5 6 2细胞株细胞相比 ,Survivin m RNA表达上调了 1.6倍。外周血单个核细胞 Survivin m RNA表达阴性。经 ATRA处理后 ,NB4细胞Survivin m RNA表达随着时间的延长而呈逐渐下降趋势 ,在 72小时时几乎检测不到 Survivin m RNA的表达。结论　 Survivin基因是肿瘤细胞抗凋亡和耐药的重要机制之一 。Objective To investigate the expression profile of Survivin gene in hematological tumour cell lines. Method The transcriptional levels of Survivin gene were detected by RT-PCR. Results Survivin gene was expressed in 11 hematological tumout cell lines(K562,HL-60,U937,HEL,6T-CEM,NB4,Jurkat,Raji,MUTZ-1,Dami and K562/ADR cell lines), in which the transcriptional levels were significantly higher than those in disease controls. In addition, there existed differential expression levels of Survivin gene in hematological tumour cell lines(NB4>MUTZ-1>K562>Raji>HL-60>6T-CEM>U937>HEL>Jurkat>Dami).The transcriptional levels of Survivin gene in K562/ADR cell line increased 1.6-fold compared with the parent K562 cell line. The Survivin expression was not detected in peripheral blood mononuclear cells. All-trans retinoic acid significantly decreased Survivin mRNA expression levels. Conclusion The Survivin gene might be a potential therapeutic target due to the role in anti-apoptosis and drug resistance.

关 键 词：SURVIVIN基因 血液肿瘤 细胞株 细胞表达 聚合酶链反应 

分 类 号：R733[医药卫生—肿瘤]