胰腺癌组织中基质金属蛋白酶-7及其组织抑制物-1的表达  

Expressions of Matrix Metalloproteinase 7 and Its Tissue Inhibitor 1 in Pancreatic Carcinoma

作　　者：李航宇[1] 王鹏[2] 宋茂民[3] 郭仁宣[1] 李庆昌[4] 刘忠[5] 刘博[6] 

机构地区：[1]中国医科大学附属第一医院外科,辽宁沈阳110001 [2]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001 [3]北京天坛医院普外科 [4]中国医科大学基础医学院病理学教研室 [5]沈阳市第七人民医院普外科 [6]鞍山市鞍钢医院普外科

出　　处：《中国医科大学学报》2003年第6期553-555,共3页Journal of China Medical University

摘　　要：目的 :观察胰腺及其癌变组织中基质金属蛋白酶 7(MMP 7)及其组织抑制物 1(TIMP 1)蛋白的表达 ,以探讨MMP 7和TIMP 1在胰腺癌浸润转移中的作用。方法 :用免疫组化染色方法和显微图像分析技术检测 32例胰腺癌石蜡包埋组织和 4例正常胰腺组织中MMP 7及TIMP 1的表达。结果 :正常组织中MMP 7和TIMP 1的平均积分光密度分别为 (8.2 4± 7.14 )和 (5 .94± 2 .79) ,与癌组织相比差异显著 ,P <0 .0 1。在伴有淋巴结或肝转移的胰腺癌组织中MMP 7和TIMP 1的平均积分光密度分别为 (6 8.5 7± 31.6 5 )和 (5 2 .86± 33.4 7)。在没有淋巴结或肝转移的胰腺癌组织中MMP 7和TIMP 1的平均积分光密度分别为 (43.0 3± 36 .12 )和 (2 9.19± 2 6 .5 7)。两组比较差异显著 ,P <0 .0 5。结论 :MMP 7和TIMPObjective: Our aims were to evaluate the expressions of metalloproteinase 7 and tissue inhibitors of metalloproteinase 1 in human pancreatic tissue and pancreatic carcinoma tissue and to investigate the role of MMP 7 and TIMP 1 in the progression, transformation and invasiveness of human pancreatic carcinoma. Methods: A total of 32 cases of pancreatic carcinoma and 4 of normal pancreatic tissue, were tested for expressions of MMP 7 and TIMP 1 by using immunohistochemical methods and image analysis technique. Results: The integrated optical density averages of MMP 7 and TIMP 1 in normal human pancreatic tissue were significantly lower than those in pancreatic carcinoma tissue (P<0.01). In cases with lymph node or liver metastasis, the averages were (68.57±31.65) and (52.86±33.47), which were significantly higher than (43.03±36.12) and (29.19±26.57) in those without metastasis ( P<0.05). Conclusion: The increased expression of MMP 7 and TIMP 1 were related to the progression of pancreatic carcinoma and might indicate the biological aggressiveness and malignancy of pancreatic carcinoma.

关 键 词：胰腺肿瘤 基质金属蛋白酶 免疫组织化学 图像分析 

分 类 号：R735.9[医药卫生—肿瘤]