免疫表型分型结合FISH检测自然杀伤细胞淋巴瘤细胞的染色体畸变  

The Detection of Chromosome Aberrations in Natural Killer Cell Lymphoma/leuk1emia with a Combination of Immunophenotyping and FISH

作　　者：翁立红[1] 周俊[1] 张颜明[1] 顾文祥[1] 

机构地区：[1]浙江大学医学院,浙江杭州310031

出　　处：《浙江大学学报（医学版）》1999年第6期253-256,267,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省教委科研基金

摘　　要：目的：检测自然杀伤（ＮＫ）细胞淋巴瘤中含有染色体畸变的肿瘤细胞的免疫表型。方法：采用免疫表型分型结合荧光原位杂交（ＦＩＳＨ）技术，在单细胞水平检测了３例ＮＫ细胞淋巴瘤中含有染色体畸变的肿瘤细胞的免疫表型。结果：在该３例中，克隆性的染色体畸变仅见于ＣＤ５６＋ 细胞，在ＣＤ３＋ 细胞和ＣＤ５＋ 细胞中未发现相应染色体畸变。结论：实验表明，ＮＫ细胞淋巴瘤中，含有染色体畸变的肿瘤细胞局限于ＣＤ５６＋ 、ＣＤ３－ 和ＣＤ５－ 的ＮＫ细胞群。Objective:To detect the immunophenotypes of tumor cells with chromosome aberrations in three cases of natural killer (NK) cell lymphoma/leukemia.Methods:A combination of immunophenotyping and FISH.Results:In all three cases,clonal chromosome aberrations were detected only in CD56+ cells,not in CD3+ or CD5+ cells.Conclusion:This study indicates that chromosome aberrations in NK cell lymphoma/leukemia might be restricted to the CD56+,CD3- and CD5- cell populations and that NK cell lymphoma/leukemia may derive from mature NK cells and not from T lymphocytes.

关 键 词：淋巴瘤 非霍奇金氏/病因学 杀伤细胞 天然 免疫表型分型 染色体畸变 原位杂交 荧光 抗原 CD56/分析 

分 类 号：R733.1[医药卫生—肿瘤]