玉米大斑病菌毒素对玉米叶肉细胞脂氧合酶活性的影响  

Effects of HT-toxin produced by Exserohilum turcicum on lipoxygenase activity in corn leaf cells

作　　者：张建成[1] 范永山[1] 董金皋[1] 

机构地区：[1]河北农业大学真菌毒素实验室,保定071001

出　　处：《植物病理学报》2003年第5期421-424,共4页Acta Phytopathologica Sinica

基　　金：国家教育部骨干教师资助计划;河北省自然科学基金项目 (30 2 31 8)

摘　　要：玉米大斑病菌 2号小种粗毒素 (HT C)和标准毒素 (5 羟甲基 2 呋喃甲醛 ,HT I)处理玉米叶片 ,都能诱导 3种供试基因型玉米叶片中脂氧合酶活性增高 ,但是不同基因型玉米对HT C和HT I的反应不同 ,HT I对含有Ht1基因的品系诱导时间早 ,水平高 ,HT C对于不带Ht基因的品系LOX的影响略高于HT I;不同浓度的HT I在OH4 3和OH4 3Ht1玉米叶片中诱导的LOX酶活性不同 ,高浓度HT I和HT C对LOX酶活诱导时间早 ,水平高 ,浓度降低诱导水平降低 ;外源H2 O2 对HT I和HT C在OH4Effects of toxic crude culture filtrate (HT-C) from Exserohilum turcicum race 2 and standard HT-I fraction (5-hydroxymethyl-2-furancarboxaldehyde)on the activity of lipoxygenase were compared among leaf cells of corn inbreds with different Ht gene.In contrast to standard fraction,the peak of lipoxygenase activity induced by HT-toxin came out lower and later.It was also revealed that they had different effects depend on different corn resistant genotypes.The activity of the enzyme in corn leaf cells with Ht2 gene was higher and earlier when treated with HT-toxin than with standard fraction.But for corn leaf cells with Ht1 gene,the results were completely different.The effects of HT-toxin were a little higher in corn leaf cells without any Ht resistant genes.The activity of the enzyme changed according to their concentration.Higher concentration,higher the activity and earlier appearing the peak.Adding exogenous H 2O 2,the effects of HT-toxin and standard fraction on the inducing activity of lipoxygenase changed so dramatically that they were dispensable.

关 键 词：玉米 大斑病菌 叶肉细胞 脂氧合酶 活性 毒素 HT-C 5-羟甲基-2-呋喃甲醛 HT-I 基因 H202 浓度 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]