Extract from Fructus cannabis activating calcineurin improved learning and memory in mice with chemical drug-induced dysmnesia  

对钙调神经磷酸酶有激活作用的火麻仁提取物改善化学药品诱发的小鼠学习记忆功能障碍(英文)

作　　者：骆静[1] 尹江华[1] 吴和珍[1] 魏群[1] 

机构地区：[1]北京师范大学生命科学学院生物化学及分子生物学系

出　　处：《Acta Pharmacologica Sinica》2003年第11期1137-1142,1175,共7页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (№ 39930060) and the State Key Basic Research and Development Program (№ 1998051123).

摘　　要：AIM: To investigate the effects of extract from Fructus cannabis(EFC) that can activate calcineurin on learning and memory impairment induced by chemical drugs in mice. METHODS: Bovine brain calcineurin and calmodulin were isolated from frozen tissues. The activity of calcineurin was assayed using p-nitrophenyl phosphate(PNPP) as the substrate. Step-down type passive avoidance test and water maze were used together to determine the effects of EFC on learning and memory dysfunction. RESULTS: EFC activated calcineurin activity at a concentration range of 0.01-100g/L. The maximal Value of EFC on calcineurin activity(35%±5%) appeared at a concentration of 10g/L. The chemical drugs such as scopolamine, sodium nitrite, and 45% ethanol, and sodium pentobarbital induced learning and memory dysfunction. EFC administration(0.2, 0.4, and 0.8g/kg, ig×7d) prolonged the latency and decreased the number of errors in the step-down test. EFC, given for 7d, enhanced the spatial resolution of amnesic mice in water maze test. EFC overcome amnesia of three stages of memory process at the dose of 0.2g/kg. CONCLUSION: EFC with an activation role of calcineurin can improve the impaired learning and memory induced by chemical drugs in mice.目的:观察对钙调神经磷酸酶有激活作用的火麻仁提取物对化学药品诱发的小鼠学习记忆功能障碍的改善作用。方法:以对硝基苯酚磷酸盐为底物,测定了火麻仁提取物对钙调神经磷酸酶的作用,利用东莨菪碱、亚硝酸钠、乙醇和戊巴比妥钠造成小鼠学习记忆障碍,学习记忆功能检测采用小鼠跳台法和水迷宫法。结果:以牛脑中提取的钙调神经磷酸酶的比活力为100％,火麻仁提取物的浓度在10g/L时,对牛酶的最大激活达到35％±5％。化学药品如东莨菪碱、亚硝酸钠、45％乙醇和戊巴比妥钠均引起小鼠学习记忆功能障碍,主要表现为在跳台作业中潜伏期短,累计错误次数增多;在水迷宫中也同样出现了小鼠空间学习记忆障碍,用对钙调神经磷酸酶有激活作用的火麻仁提取物(0.2,0.4,0.8g/kg)连续给予(ig) 7 d,分别对上述改变有不同程度的改善作用。结论:对钙调神经磷酸酶有激活作用的火麻仁提取物能够改善化学药品诱发的小鼠学习记忆功能障碍。

关 键 词：Fructus cannabis CALCINEURIN avoidance learning maze learning memory disorders 

分 类 号：R285[医药卫生—中药学]