猪胰羧肽酶B的分离与纯化  

Separation and Purification of Carboxypeptidase B in Porcine Pancreas

作　　者：卢映霞[1] 邹静恂[1] 茹炳根[2] 卢军[2] 刘德富[3] 

机构地区：[1]首都医学院化学教研室 [2]北京大学生物系 [3]北京大学分校

出　　处：《首都医学院学报》1992年第1期10-14,共5页

摘　　要：改进了从猪胰中提取羧肽酶B的方法,使回收率提高到15％,比活性增加到192U/mg蛋白,酶的纯度达到91％。For enhancing the purification recovery of carboxypeptidase B, the Felk' s method was modified as following: (1)Both of Tris-HCl buffers with contained 0. 1 mol/L and 0. 15 mol/L NaCl were ap- plied respectively in the batch adsorption and elution from DEAE-cellulose. (2)In the second DEAE-cellulose chromatography, the DEAE-cellulose column was e- luted with linear gradient of NaCl from 0.00 to 0.09 mol/L in Tris-HAc buffer. (3)Carboxypeptidase B was eluted completely with 0. 09 mol/L NaCl in Tris-HAc buffer. The purity and the molecular weight were identified by TLC scanning method after gel electrophoresis. The results showed that recovery of enzyme activity was in creased from 8% to 15%, specific activity was increased from 175 U/mg to 192 U/mg and purity of en- zyme was reached 91%.

关 键 词：羧基肽酶B 猪胰 分离 纯化 

分 类 号：TQ464.8[化学工程—制药化工]