皮质酮对大鼠再生肝细胞转录活性的影响  

Effects of corticosterone on transcriptional activity of regenerating hepatocytes in the rat

作　　者：宁黔冀[1] 翟心慧[1] 李恩中[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453002

出　　处：《动物学报》2003年第5期615-619,共5页ACTA ZOOLOGICA SINICA

基　　金：河南省自然科学基金(No . 2 0 0 1180 0 0 09);河南省高校青年骨干教师资助计划;河南省重点学科资助~~

摘　　要：以AgNOR颗粒数为指标 ,研究大鼠部分肝切除后 ,皮质酮对余留肝细胞转录活性的影响。结果显示 :部分肝切除后 0～ 2 4h ,各组肝细胞内 (假手术、去肾上腺、去肾上腺 +皮质酮 )AgNOR颗粒数均下降 ;部分肝切除后 36h ,假手术鼠的AgNOR数目最多 ,到 4 8h时已基本恢复到肝切除前水平 ;在部分肝切除后 2 4～ 4 8h ,去肾上腺鼠的AgNOR颗粒数持续升高 ;给去肾上腺鼠再注射剂量分别为 10、 2 0、 4 0mg/kg体重的皮质酮 ,发现在 36h和 4 8h时 ,皮质酮剂量越高 ,AgNOR颗粒数目越少 ,且下降幅度越大。部分肝切除前 12h给大鼠注射糖皮质激素受体颉颃剂———RU4 86 ( 10mg/kg体重 ) ,结果与去肾上腺鼠相似。以上结果表明 :皮质酮对部分肝切除后肝细胞的转录活性具有明显的抑制作用 ,而且是通过受体起作用 ,该作用表现在部分肝切除 2 4h之后 。The effect of corticosterone on transcriptional activity in regenerating hepatocytes of rat after partial hepatectomy (PH) was studied by using nucleolar organizer region associated proteins (AgNOR) staining as marker. The results showed that: during 0-24 h after PH, the numbers of AgNOR decreased whatever in sham adrenalectomized rats, adrenalectomized rats or adrenalectomized rats treated with corticosterone. The numbers of AgNOR in sham operated rats peaked at 36 h after PH, and then restored the pre PH level at 48 h. AgNORs increased constantly 24-48 h after PH in adrenalectomized rats. After the treatment of corticosterone at a dose of 10 or 20 or 40 mg/kg body weight respectively in the adrenalectomized rats, it was found that at 36 h and 48 h post PH, the more corticosterone used, the less the AgNORs appeared and the faster the ranger of AgNORs declined. RU486, the glucocorticoid receptor antagonist, was given subcutaneously at a dose of 10 mg/kg body weight 12 h before PH in order to investigate extensively the action of endogenous corticosterone. The similar results were observed in the adrenalectomized rats. These findings suggested that transcriptional activity in regenerating hepatocytes of rat was inhibited by corticosterone. This phenomenon appeared mainly at 24 h following PH via the binding of corticosterone to its receptor.

关 键 词：大鼠 肝切除 皮质酮 转录活性 

分 类 号：Q57[生物学—生物化学]