应用双抗体夹心ELISA法定量检测人源破伤风毒素单克隆抗体中的IgG含量  

A sandwich enzyme immunoassay for quantitative detection of IgG contents of anti-tetanus human monoclonal antibodies

作　　者：赵红[1] 桥爪秀一 王棣[1] 李晓进[1] 龟井优德 林卫[1] 王亚男[1] 马瑞[1] 

机构地区：[1]兰州生物制品研究所,兰州730046 [2]日本森永株式会社生物科学研究所

出　　处：《微生物学免疫学进展》2003年第4期44-46,共3页Progress In Microbiology and Immunology

摘　　要：采用山羊抗人IgG作为包被抗体 ,辣根过氧化物酶标记的山羊抗人IgG作为标记抗体 ,建立一种双抗体夹心法用于定量检测人源破伤风毒素单克隆抗体的IgG含量。以纯化的IgG作标准 ,用平行线法测得亲和层析纯化的人源破伤风毒素单克隆抗体G2的含量为 0 .5 12 μg/ml,与分光光度法测得的结果基本一致。因而样品检测采用纯化G2作参考标准 ,制作标准曲线 ,测定了已知样品和未知样品的抗体含量。结果表明本法重复性好 ,特异性强 。A sandwich enzyme immunoassay for quantitative detection of IgG contents of anti tetanus human monoclonal antibodies was developed by using goat anti human IgG as coating antibody and horseradish peroxidase (HRPO) labelled goat anti human IgG as labelled antibody. The concentration of purified G2 with anti tetanus toxin human monoclonal antibody was 0.512μg/ml, which was determined by comparing the parallel dilution curves of samples with that of standard IgG. Antibody contents of some known and unknown samples were detected by comparing dilution samples with purified G2 as a reference . The results suggested the sandwich ELISA assay was specific and repetitive .It is suitable for quantitative detection of IgG contents of human monoclonal antibodies both in the culture and purification processes.

关 键 词：人源破伤风毒素单克隆抗体 双抗体夹心ELISA法 山羊抗人IgG 定量检测 含量检测 

分 类 号：R392[医药卫生—免疫学]