基因工程菌生产rhG-CSF发酵培养基的研究  

Studies on Culture Media of Escherichia coli Producing rhG-CSF

作　　者：王海波[1] 欧俊杰[2] 耿信笃[2] 

机构地区：[1]中国人民解放军第四军医大学化学教研室,西安710032 [2]西北大学现代分离科学研究所陕西省重点实验室,西安710069

出　　处：《中国生物工程杂志》2003年第11期68-71,共4页China Biotechnology

基　　金：国家自然科学基金资助项目 ( 3 9880 0 0 3 )

摘　　要：在摇瓶中对工程菌DH5α pBV2 2 0生产rhG CSF的发酵培养基进行了研究 ,从几种常用的细菌培养基中筛选适合工程菌表达rhG CSF的M9培养基。利用正交试验优化出生产重组人rhG CSF的优化培养基配方 ,其中优化碳源含量为 2 %的葡萄糖 ,氮源为 2 5 %的酵母粉、3%的蛋白胨和 0 1 %的 (NH4) 2 SO4以及少量的无机盐。使用优化培养基可使rhG CSF的表达量达到33 8% ,比优化前提高了 48 9%。The fermentation culture media for recombinant E.coli DH5α/pBV220 was investigated with shake culture method.The M9 mediums elected from several kinds of basic culture media was finally used.The optimized culture medium was obtained by orthogonal method.Optimized prescription of culture medium was investigated for the fermentation of E.coli to produce recombinant rhG CSF.The optimum culture medium consisting of 2% glucose,2.5%yeast,3%tryptone,and 0.1% (NH 4) 2SO 4 .The expression of recombinant rhG CSF was 33.8% with the optimum culture medium,which had increased by 48.9% in comparison with previous yield.

关 键 词：重组人粒细胞集落刺激因子 重组大肠杆菌 发酵培养基 表达量 

分 类 号：TQ929[轻工技术与工程—发酵工程] Q78[生物学—分子生物学]